Timp1 (NM_011593) Mouse Recombinant Protein

CAT#: TP723887

Purified recombinant protein of Mouse tissue inhibitor of metalloproteinase 1 (Timp1), transcript variant 2

Product Images

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Mouse TIMP-1, the region of Cys27-Arg205, from gene Accession# NM_011593
• Tag: C-His
• Predicted MW: 22 kDa
• Concentration: lot specific
• Purity: >90%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0
• Bioactivity: Mouse TIMP-1 inhibits the activity of activated mouse MMP-9 (100 ng/mL). The IC50 = 90 ng/mL.
• Endotoxin: Less than 1.0 EU per µg of protein as determine by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_035723
• Locus ID: 21857
• UniProt ID: P12032
• Cytogenetics: X 16.38 cM
• Refseq Size: 902
• Refseq ORF: 615
• Synonyms: Clgi; EPA; Timp; TIMP-1; TPA-S1
• Summary: Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14 (By similarity). Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.[UniProtKB/Swiss-Prot Function]