CD40L (CD40LG) (NM_000074) Human Recombinant Protein

SKU TP723872

Purified recombinant protein of Human CD40 ligand (CD40LG / TNFSF5)

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Human CD40L, the region of Glu108-Leu261, from gene Accession# NM_000074.2
• Tag: N-His
• Predicted MW: 19 kDa
• Concentration: lot specific
• Purity: >95%, as determined by Coomassie stained SDS-PAGE
• Buffer: 1 x PBS
• Bioactivity: Human CD40L induced the proliferation of human B cells in the presence of IL-4 in a dose-dependent manner. The ED50 for this effect is 6 – 36 ng/mL.
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_000065
• Locus ID: 959
• UniProt ID: P29965
• Cytogenetics: Xq26.3
• RefSeq Size: 1834
• RefSeq ORF: 783
• Synonyms: CD40L; CD154; gp39; hCD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP
• Summary: The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Allograft rejection, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Cytokine-cytokine receptor interaction, Primary immunodeficiency, Systemic lupus erythematosus, T cell receptor signaling pathway, Viral myocarditis