TNFRSF1B (NM_001066) Human Recombinant Protein

CAT#: TP723871

Purified recombinant protein of Human tumor necrosis factor receptor superfamily, member 1B (sTNF-RII / TNFRSF1B)

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Human TNF-RII, the region of Pro 24-Thr206 with an N-terminal Met, from gene Accession# AAA36755
• Tag: Tag Free
• Predicted MW: 20 kDa
• Concentration: lot specific
• Purity: >95%, as determined by Coomassie stained SDS-PAGE
• Buffer: 1 x PBS
• Bioactivity: The ED50 is 0.15-0.5 µg/ml, corresponding to a specific activity of 2-6 x 103 units/mg, determined by a dose-dependent inhibition of 0.25 ng/ml TNF-a induced cytotoxicity in L929 mouse fibroblast cells in the presence of 4 µg/ml actinomycin D.
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_001057
• Locus ID: 7133
• UniProt ID: P20333
• Cytogenetics: 1p36.22
• Refseq Size: 3682
• Refseq ORF: 1383
• Synonyms: CD120b; p75; p75TNFR; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR1B; TNFR2; TNFR80
• Summary: The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Amyotrophic lateral sclerosis (ALS), Cytokine-cytokine receptor interaction