IL2 (NM_000586) Human Recombinant Protein

CAT#: TP723850

Purified recombinant protein of Human interleukin 2 (IL2)

Specifications

Product Data

• Description: Purified recombinant protein of Human interleukin 2 (IL2)
• Species: Human
• Expression Host: Sf9
• Expression cDNA Clone or AA Sequence: Human IL-2, the region of Ala21-Thr153, from gene Accession# NM_000586
• Tag: Tag Free
• Predicted MW: 15.4 kDa
• Concentration: Protein formulated and vialed at >50 ug/mL in specified buffer
• Purity: Purity is >95%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 10mM NaH2PO4, pH7.2, 150mM NaCl.
• Bioactivity: The ED50 is 0.5 - 2.0 ng/ml, corresponding to a specific activity of 0.5 - 2.0x10^6 units/mg, determined by the dose dependent stimulation of CTLL2 cell proliferation.
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_000577
• Locus ID: 3558
• Refseq Size: 822
• Cytogenetics: 4q27
• Refseq ORF: 459
• Synonyms: IL-2; lymphokine; TCGF
• Summary: The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Allograft rejection, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Jak-STAT signaling pathway, T cell receptor signaling pathway, Type I diabetes mellitus