Il23a (NM_031252) Mouse Recombinant Protein

CAT#: TP723849

Purified recombinant protein of Mouse interleukin 23, alpha subunit p19 (Il23a)

Product Images

Specifications

Product Data

• Species: Mouse
• Expression Host: Sf9
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Mouse IL-23 consists of two subunits: P19, the region of Met1-Ala196 from gene Accession# NM_031252, and P40, the region of Met1-Ser 335 from gene Accession# NM_008352, linked via a disulphide bond:
• Tag: Tag Free
• Predicted MW: 55.4 kDa
• Concentration: lot specific
• Purity: Purity is >95%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 1 x PBS
• Bioactivity: The ED50 is 0.5 - 0.8 ng/ml, corresponding to a specific activity of 1.25-2.0 x 10^6 units/mg, determined by mouse splenocytes IL-17A secretion induced by mIL-23 in a dose dependent manner. Under this assay, the bioactivity is equivalent
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_112542
• Locus ID: 83430
• UniProt ID: Q9EQ14
• Cytogenetics: 10 D3
• Refseq Size: 1359
• Refseq ORF: 591
• Synonyms: IL-23; p19
• Summary: Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.[UniProtKB/Swiss-Prot Function]