Egf (NM_010113) Mouse Recombinant Protein

CAT#: TP723822

Purified recombinant protein of Mouse epidermal growth factor (Egf)

Specifications

Product Data

• Species: Mouse
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Mouse EGF, 53 the region of Asn977-Arg1029, from gene Accession# NP_034243
• Tag: Tag Free
• Predicted MW: 6 kDa
• Concentration: lot specific
• Purity: >98%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 1 x PBS
• Bioactivity: The ED50 is 1-2 ng/ml, corresponding to a specific activity 5x10^5 - 1x10^6 units/mg, determined by a dose-dependent inhibition on A431 cells proliferation.
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_034243
• Locus ID: 13645
• UniProt ID: P01132, Q3UWD7
• Cytogenetics: 3 58.5 cM
• Refseq Size: 4757
• Refseq ORF: 3654
• Synonyms: AI790464
• Summary: This gene encodes epidermal growth factor (EGF), the founding member of the EGF family of growth factors that are implicated in cell proliferation and differentiation. The encoded protein can localize to the membrane and function in juxtacrine signaling or undergo proteolytic processing to generate a soluble form of the hormone. Mice lacking the encoded protein do not exhibit an abnormal phenotype but transgenic mice overexpressing the encoded protein exhibit hypospermatogenesis. [provided by RefSeq, Jul 2016]