Tnf (NM_012675) Rat Recombinant Protein

CAT#: TP723777

Purified recombinant protein of Rat tumor necrosis factor (TNF superfamily, member 2) (Tnf), (10 ug)

Product Images

The ED50 is 2-12 pg/mL, corresponding to a specific activity of 0.83-5 x 10^8 units/mg, as determined by a dose dependent cytotoxicity assay using L929 cells treated with actinomycin D.

Specifications

Product Data

• Species: Rat
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Rat TNF-a, the region of Leu80-Leu235, from gene Accession# NM_012675
• Tag: Tag Free
• Predicted MW: The 157 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of approximately 17,361 Da. The DTT-reduced protein migrates at approximately 17kDa and the non-reduced protein migrates at approximately 16kDa by SDS-PAGE.
• Concentration: lot specific
• Purity: >98%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 10 mM NaH2PO4, 150 mM NaCl, pH 7.2
• Endotoxin: Less than 0.1 EU/µg (<0.01 ng/µg) protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_036807
• Locus ID: 24835
• UniProt ID: P16599
• Cytogenetics: 20p12
• Refseq Size: 1687
• Refseq ORF: 705
• Synonyms: RATTNF; TNF-alpha; Tnfa
• Summary: acts as a cytokine; binds TNF receptors; plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response [RGD, Feb 2006]