Il10 (NM_010548) Mouse Recombinant Protein

CAT#: TP723748

Purified recombinant protein of Mouse interleukin 10 (Il10)

Product Images

Specifications

Product Data

• Species: Mouse
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Mouse IL-10, the region of Ser19-Ser178, from gene Accession# NM_010548, with a Cys149Tyr substitution
• Tag: Tag Free
• Predicted MW: 18.9 kDa
• Concentration: lot specific
• Purity: Purity is >98%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 20 mM Tris pH 7.5
• Bioactivity: The ED50 is 1- 2 ng/ml, corresponding to a specific activity of 1- 0.5 x 10^6 units/mg, determined by the dose dependent stimulation MC/9 cell proliferation.
• Endotoxin: Less than 0.01 ng per ug protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_034678
• Locus ID: 16153
• UniProt ID: P18893, Q3U879
• Cytogenetics: 1 56.89 cM
• Refseq Size: 1306
• Refseq ORF: 534
• Synonyms: CSIF; If2a; IL-; Il-10
• Summary: This gene encodes an anti-inflammatory cytokine that is a member of the class-2 cytokine family. The encoded protein is secreted by cells of both the innate and adaptive immune systems and is crucial for limiting the immune response to a broad range of pathogens. It also has been shown to suppress autoimmune responses. This protein mediates it's immunosuppressive signal through a specific interleukin 10 receptor complex. Aberrant functioning of this gene is associated with numerous immune disorders including graft-versus-host disease, and increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, Sep 2015]