Il4 (NM_021283) Mouse Recombinant Protein

CAT#: TP723734

Purified recombinant protein of Mouse interleukin 4 (Il4), transcript variant 1

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Mouse IL-4, the region of His23-Ser140, from gene Accession# NM_021283
• Tag: Tag Free
• Predicted MW: 13.3 kDa
• Concentration: lot specific
• Purity: >95%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 1 x PBS
• Bioactivity: The ED50 is 2 - 4 ng/ml, corresponding to a specific activity of 2.5 - 5 x 10^5 units/mg, determined by CTLL-2 cell proliferation induced by mouse IL-4 in a dose dependent manner.
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_067258
• Locus ID: 16189
• UniProt ID: P07750
• Cytogenetics: 11 31.97 cM
• Refseq Size: 605
• Refseq ORF: 420
• Synonyms: BSF-1; Il-4
• Summary: Participates in at least several B-cell activation processes as well as of other cell types (PubMed:3083412). It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells (PubMed:3498301). It enhances both secretion and cell surface expression of IgE and IgG1 (PubMed:3498301). It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages (PubMed:25847241). Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4 (PubMed:26416964).[UniProtKB/Swiss-Prot Function]