IL12A (NM_000882) Human Recombinant Protein

CAT#: TP723721

Purified recombinant protein of Human interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) (IL12A)

Product Images

Human IL-12 (p70) induces the production of IFNγ in human activated PBMC in a dose-dependent manner. The ED50 for this effect is 0.01 – 0.1 ng/mL.

Specifications

Product Data

• Species: Human
• Expression Host: Sf9
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Secreted protein p35: Accession# NM_000882, and p40: Accession# NM_002187
• Tag: Tag Free
• Predicted MW: 57 kDa
• Concentration: lot specific
• Purity: 95%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 10 mM NaH2PO4, 150 mM NaCl, pH 7.2
• Bioactivity: Human IL-12 (p70) induces the production of IFNγ in human activated PBMC in a dose-dependent manner. The ED50 for this effect is 0.01 – 0.1 ng/mL.
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_000873
• Locus ID: 3592
• UniProt ID: P29459, O60595
• Cytogenetics: 3q25.33
• Refseq Size: 1450
• Refseq ORF: 759
• Synonyms: CLMF; IL-12A; NFSK; NKSF1; P35
• Summary: This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Allograft rejection, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus