PLOD1 (NM_000302) Human Recombinant Protein

SKU TP701059

Purified recombinant protein of Human procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1), with C-terminal His tag, secretory expressed in HEK293 cells, 50ug

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence from TrueORF clone, [RC201969], encoding the region Lys19-Pro727 of PLOD1
• Tag: C-His
• Predicted MW: 82.9 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: PBS, pH 7.4, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_000293
• Locus ID: 5351
• UniProt ID: Q02809
• Cytogenetics: 1p36.22
• RefSeq Size: 3047
• RefSeq ORF: 2181
• Synonyms: EDS6; EDSKCL1; LH; LH1; LLH; PLOD
• Summary: Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]
• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation