Rac1 (NM_009007) Mouse Recombinant Protein
SKU
TP527648
Purified recombinant protein of Mouse Rac family small GTPase 1 (Rac1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
$1,020.00
In Stock*
Specifications
| Product Data | |
| Species | Mouse |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-MYC/DDK |
| Predicted MW | 21.9 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C after receiving vials. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_033033 |
| Locus ID | 19353 |
| UniProt ID | P63001 |
| Cytogenetics | 5 82.22 cM |
| RefSeq Size | 2284 |
| RefSeq ORF | 576 |
| Synonyms | AL023026; D5Ertd559e |
| Summary | Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states (PubMed:24352656). In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. In neurons, is involved in dendritic spine formation and synaptic plasticity (PubMed:24352656, PubMed:26969129). In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3.UniProtKB/Swiss-Prot Function |
| Protein Categories | Growth Factors, Intracellular Proteins |
Reviews
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Resources
| Recombinant Protein Resources |
Citations
Burkholderia pseudomallei BopE suppresses the Rab32-dependent defense pathway to promote its intracellular replication and virulence
Rao, Zhang, Qiao et al
mSphere (2024)
Rao, Zhang, Qiao et al
mSphere (2024)
Cited by 1
AI Summary: OriGene products Rab32 (TP502577) and Rac1 (TP527648) recombinant proteins were used in nucleotide exchange assays to investigate the interaction between BopE and host Rab32, elucidating the mechanism by which BopE suppresses Rab32-dependent defense pathways during Burkholderia pseudomallei infection.
Snippet: Nucleotide exchange assays were conducted using fluorescent N-methylanthraniloyl-GDP (Mant-GDP, Molecular Probes), following a modified method from a previous report (33). Briefly, Rab32 (Origene, TP502577) or Rac1 (Origene, TP527648) equilibrated in loading buffer (20 mmol/L HEPES-NaOH [pH 7.5], 50 mmol/L NaCl, 0.5 mmol/L MgCl2, 5 mmol/L EDTA, 1 mmol/L DTT) was loaded with 20-fold excess
Showing 1-1 of 1 papers.
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