Gli1 (NM_010296) Mouse Recombinant Protein

CAT#: TP527023

Purified recombinant protein of Mouse GLI-Kruppel family member GLI1 (Gli1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR227023 representing NM_010296
  Red=Cloning site Green=Tags(s)
  
  MFNPMTPPQVNSYSEPCCLRPLHSQGVPSMGTEGLSGLPFCHQANFMSGSQGYGAARETSSCTEGSLFPP
  PPPPRSSVKLTKKRALSISPLSDASLDLQTVIRTSPSSLVAFINSRCTSPGGSYGHLSIGTMSPSLGFPP
  QMSHQKGTSPPYGVQPCVPHDSTRGSMMLHPQSRGPRATCQLKSELDMMVGKCPEDPLEGDMSSPNSTGT
  QDHLLGMLDGREDLEREEKPEPESVYETDCRWDGCSQEFDSQEQLVHHINSEHIHGERKEFVCHWGGCSR
  ELRPFKAQYMLVVHMRRHTGEKPHKCTFEGCRKSYSRLENLKTHLRSHTGEKPYMCEQEGCSKAFSNASD
  RAKHQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAQPLSTVEPKRE
  REGGSGREESRLTVPESAMPQQSPGAQSSCSSDHSPAGSAANTDSGVEMAGNAGGSTEDLSSLDEGPCVS
  ATGLSTLRRLENLRLDQLHQLRPIGSRGLKLPSLTHAGAPVSRRLGPPVSLDRRSSSSSSMSSAYTVSRR
  SSLASPFPPGTPPENGASSLPGLTPAQHYMLRARYASARGSGTPPTAAHSLDRMGGLSVPPWRSRTEYPG
  YNPNAGVTRRASDPARAADHPAPARVQRFKSLGCVHTPPSVATGRNFDPHHPTSVYSPQPPSITENVAMD
  TRGLQEEPEVGTSVMGNGLNPYMDFSSTDTLGYGGPEGTAAEPYEARGPGSLPLGPGPPTNYGPGHCAQQ
  VSYPDPTPENWGEFPSHAGVYPSNKAPGAAYSQCPRLEHYGQVQVKPEQGCPVGSDSTGLAPCLNAHPSE
  GSPGPQPLFSHHPQLPQPQYPQSGPYPQPPHGYLSTEPRLGLNFNPSSSHSTGQLKAQLVCNYVQSQQEL
  LWEGRNRGGLPNQELPYQSPKFLGGSQVSQSPAKTPAAAAAAYGSGFAPASANHKSGSYPAPSPCHETFT
  VGVNRPSHRPAAPPRLLPPLSPCYGPLKVGDTNPSCGHPEVGRLGAGPALYPPPEGQVCNALDSLDLDNT
  QLDFVAILDEAQGLSPPLSHEQGDSSKNTPSPSGPPNMAVGNMSVLLGSLPGETQFLNSSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 119 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_034426
• Locus ID: 14632
• UniProt ID: P47806
• Cytogenetics: 10 74.5 cM
• Refseq Size: 3662
• Refseq ORF: 3333
• Synonyms: AV235269; Zfp-5; Zfp5
• Summary: Acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. Regulates the transcription of specific genes during normal development. Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling. Plays a role in cell proliferation and differentiation via its role in SHH signaling.[UniProtKB/Swiss-Prot Function]