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Ern1 (NM_023913) Mouse Recombinant Protein

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SKU
TP526999
Purified recombinant protein of Mouse endoplasmic reticulum (ER) to nucleus signalling 1 (Ern1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Size:
20 ug
  $1,020.00
4 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Mouse
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>MR226999 representing NM_023913
Red=Cloning site Green=Tags(s)

MPARWLLLLLALLLPPPGPGSFGRTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHV
EEPAFLPDPNDGSLYTLGGKNNEGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQT
LSSAFADSLCPSTSLLYLGRTEYTITMYDTKTRELRWNATYFDYAASLPEDDVDYKMSHFVSNGDGLVVT
VDSESGDVLWIQNYASPVVAFYVWQGEVLRKVVHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKS
KLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDLKFDPGLKG
KSKLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKRSFEEVINIVGQTSDNTPTTV
SQDVEEKLARAPAKPEAPVDSMLKDMATIILSTFLLVGWVAFIITYPLSVHQQRQLQHQQFQKELEKIQL
LQQQQLPFHPHGDLTQDPEFLDSSGPFSESSGTSSPSPSPRASNHSLHPSSSASRAGTSPSLEQDDEDEE
TRMVIVGKISFCPKDVLGHGAEGTIVYKGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFC
TEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLHQTTSGLAHLHSLNIVHRDLKPHNILLSMPN
AHGRIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKDNPTYTVDIFSAGCVFYYVISE
GNHPFGKSLQRQANILLGACNLDCFHSDKHEDVIARELIEKMIAMDPQQRPSAKHVLKHPFFWSLEKQLQ
FFQDVSDRIEKEALDGPIVRQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDLLRAMRNKKHH
YRELPVEVQETLGSIPDDFVRYFTSRFPHLLSHTYQAMELCRHERLFQTYYWHEPTEPQPPVIPYAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-MYC/DDK
Predicted MW 110.6 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_076402
Locus ID 78943
UniProt ID Q9EQY0
Cytogenetics 11 E1
RefSeq Size 3976
RefSeq ORF 2931
Synonyms 9030414B18Rik; AI225830; C85377; Ire1a; Ire1alpha; Ire1p
Summary Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed:11850408, PubMed:25164867). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed:25164867). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed:11850408, PubMed:25164867). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed:11850408, PubMed:25164867). Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A (By similarity).UniProtKB/Swiss-Prot Function
Protein Categories Enzyme: Kinases, Growth Factors, Intracellular Proteins, Membrane Proteins
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Documents
Resources

file-alt Citations

Inositol Requiring Enzyme 1 Facilitates Diabetic Wound Healing through Modulating microRNAs
Wang, Qiu, Yang et al
Diabetes (2016)
Cited by 69
AI Summary: OriGene’s pCMV-miR vector and pre-miR-200 and pre-miR-466 constructs were used to express specific microRNAs in the study, enabling the investigation of how these microRNAs, regulated by IRE1α, affect angiogenesis and wound healing in diabetic conditions.
Snippet: expressing pre-miR-200 or pre-miR-466 were constructed by inserting human pre-miR-200 and pre-466 sequence into pCMV-miR vector between SgfI and MluI site (Origene). ... 8 from Origene) were used as template for PCR amplification of the linearized DNAs containing ... Cite SaveSaving
Hypothermia and Rewarming Activate a Macroglial Unfolded Protein Response Independent of Hypoxic-Ischemic Brain Injury in Neonatal Piglets
Lee, Wang, Reyes et al
Dev. Neurosci. (2016)
Cited by 29
AI Summary: OriGene's recombinant ERN1 protein was used as a positive control to validate ERN1 antibody specificity in piglet tissue experiments in this study.
Snippet: Mouse monoclonal IgG anti-ERN1 (1:200, Abgent, San Diego, Calif., USA) was used. We tested several ERN1 antibodies in piglet tissue for comparison to a positive control recombinant ERN1 protein (Origene, Rockville, Md., USA). ... Cite SaveSaving
Showing 1-2 of 2 papers.
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