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Phlpp1 (NM_133821) Mouse Recombinant Protein

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SKU
TP526995
Purified recombinant protein of Mouse PH domain and leucine rich repeat protein phosphatase 1 (Phlpp1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Size:
20 ug
  $1,020.00
4 Weeks*
Bulk/Customize

Frequently Bought Together (1)

TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications
Specifications
Product Data
Species Mouse
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>MR226995 representing NM_133821
Red=Cloning site Green=Tags(s)

MEPAAAAPAQRLADPTGEDQALAAAAAEGGRCPDPALSAAAPSGGNGGAAREEAPCEAPPGPLPGRAGGT
GRRRRRGAPQPAAGGAAPVPAAGGGANSLLLKRGRLKRNLSAAAAASSSSSPSSASSAAGGLPASCSASA
SLCTRSLDRKTLLLKHRQLLQLQPSDRDWVRHQLQRGCVHVFDRHMASSYLRPVLCTLDTTAAEVAARLL
QLGHKGGGVVKVLGYGPPPAAAPAASDQTLDGEHGRDVEPPPSSGTVGAVRGPARAPPADLPLPGGAWTR
CAPRISPAPSDSSPGELFAGGPGSPPRAPRPASDTESFSLSPSAESVSDRLDPYSSGGGGSSSSSEELEA
DPAMPHRPGRPAQPRPPSPKTSALLQPKAPTGVDSTGVIAGEGPGDDKAMAAAAPDVPLSTSGRIRETVQ
KTSPPSLYVQLHGETTRRLEADEKPLQIQNDYLFQLGFGELWRVQEEGMDSEIGCLIRFYAGKPHSTGSS
ERIQLSGMYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSVSGKMHVLPLIGGKVEEVKKHQHCLAF
SSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRISSVDLSCCSLEHLPANLFYSQDLTHLNLKQNFLRQ
TPTLPAARGLGELQRFTKLKSLNLSNNHLGAFPSAVCSIPTLAELNVSCNALREVPAAVGDMQNLQTFLL
DGNFLQSLPAELESMHQLSYLGLSFNEFTDIPEVLEKLTAVDKLCMAGNCVETLRLQALRRMPHIKHVDL
RLNILRKLMADEVDFVQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERNQLVTLNVCGYFLKALYASSNEL
AQLDVYPVPNYLSYMDVSRNCLESVPEWVCESRKLEVLDIGHNQICELPARLFCNSSLRKLLAGHNRLAR
LPERLERTSVEVLDVQHNQITELPPNLLMKADSLRFLNASANKLETLPPATLSEETSSILQELYLTNNCL
TDKCVPLLTGHPRLKILHMAYNRLQSFPASKMAKLEELEEIDISGNKLKAIPTTIMNCRRMHTVIAHSNC
IEVFPEVMQLPEVKCVDLSCNELSEITLPENLPPKLQELDLTGNPRLALDHKSLELLNNIRCFKIDQPSA
GDASGAPAVWSHGYTEASGVKNKLCVAALSVNNFRDNREALYGVFDGDRNVEVPYLLQCTMSDILAEELQ
KTKNEEEYMVNTFIVMQRKLGTAGQKLGGAAVLCHIKPDPVDLGGSFTLTSANVGKCQTVLCRNGKPLSL
SRSYIMSCEEERKRIKQHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSK
GLWDSLSIDEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDSFCCCELSAGGSMPPPS
PGIFPPSVNMVIKDRPSDGLGVPSSSSGMASEISSELSTSEMSSEVGSTASDEPPSGVLNESSPAYPNEQ
RCMLHPVCLSNSFQRQLSSATFSSAFSDNGLDSDDEEPIEGVFSNGSRVEVEVDIHCSRAKEKERQQHLL
QVPAEASDEGIVISANEDESGLSKKADFSAVGTIGRRRANGSVAPQERSHNVIEVAADAPLRKPGGYFAA
PAQPDPDDQFIIPPELEEEVKEIMKHHQEQQQQQQQQQLPPPPQPPQPQPQPQPQPQPQPQRHFQMDHLP
DCYDTPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-MYC/DDK
Predicted MW 182.8 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_598582
Locus ID 98432
UniProt ID Q8CHE4
Cytogenetics 1 E2.1
RefSeq Size 6123
RefSeq ORF 5061
Synonyms AI836256; mKIAA0606; Phlpp; Plekhe1; SCOP
Summary Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA (By similarity). Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons (By similarity). Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and suppression of tumor growth. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylates RAF1 inhibiting its kinase activity. May act as a negative regulator of K-Ras signaling in membrane rafts (By similarity). Involved in the hippocampus-dependent long-term memory formation (PubMed:17382888). Involved in circadian control by regulating the consolidation of circadian periodicity after resetting (PubMed:20080691). Involved in development and function of regulatory T-cells (PubMed:21498666).UniProtKB/Swiss-Prot Function
Protein Categories Intracellular Proteins
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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