Msh5 (NM_001146215) Mouse Recombinant Protein

CAT#: TP526215

Purified recombinant protein of Mouse mutS homolog 5 (Msh5), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Product Images

Purified recombinant protein Msh5 was analyzed by SDS-PAGE gel and Coomossie Blue Staining.

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR226215 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAFRATPGRTPPGPGPRSGIPSASFPSPQPPMAGPGGIEEEDEEEPAEIHLCVLWSSGYLGIAYYDTSDS
  TIHFMPDAPDHESLKLLQRVLDEINPQSVVTSAKQDEAMTRFLGKLASEEHREPKGPEIILLPSVDFGPE
  ISKQRLLSGNYSFISDSMTATEKILFLSSIIPFDCVLTVRALGGLLKFLSRRRIGVELEDYDVGVPILGF
  KKFVLTHLVSIDQDTYSVLQIFKSESHPSVYKVASGLKEGLSLFGILNRCRCKWGQKLLRLWFTRPTREL
  RELNSRLDVIQFFLMPQNLDMAQMLHRLLSHIKNVPLILKRMKLSHTKVSDWQVLYKTVYSALGLRDACR
  SLPQSIQLFQDIAQEFSDDLHHIASLIGKVVDFEESLAENRFTVLPNIDPDIDAKKRRLIGLPSFLTEVA
  QKELENLDSRIPSCSVIYIPLIGFLLSIPRLPFMVEASDFEIEGLDFMFLSEDKLHYRSARTKELDTLLG
  DLHCEIRDQETLLMYQLQCQVLARASVLTRVLDLASRLDVLLALASAARDYGYSRPHYSPCIHGVRIRNG
  RHPLVELCARTFVPNSTDCGGDQGRVKVITGPNSSGKSIYLKQVGLITFMALVGSFVPAEEAEIGVIDAI
  FTRIHSCESISLGLSTFMIDLNQVAKAVNNATEHSLVLIDEFGKGTNSVDGLALLAAVLRHWLALGPSCP
  HVFVATNFLSLVQLQLLPQGPLVQYLTMETCEDGEDLVFFYQLCQGVASASHASHTAAQAGLPDPLIARG
  KEVSDLIRSGKPIKATNELLRRNQMENCQALVDKFLKLDLEDPTLDLDIFISQEVLPAAPTIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 92.6 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_001139687
• Locus ID: 17687
• UniProt ID: Q9QUM7, A0A0R4IZY8
• Cytogenetics: 17 18.57 cM
• Refseq Size: 2693
• Refseq ORF: 2502
• Synonyms: Mut5
• Summary: This gene encodes a member of the MutS family of proteins that play critical roles in DNA mismatch repair and meiotic homologous recombination processes. Mice lacking the encoded protein are viable but sterile, with severe defects in spermatogenesis in males and complete loss of ovarian structures in females. Mutations in a similar gene in humans have been shown to cause common variable immune deficiency (CVID) and immunoglobulin A deficiency. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2015]