Asph (NM_023066) Mouse Recombinant Protein

CAT#: TP523585

Purified recombinant protein of Mouse aspartate-beta-hydroxylase (Asph), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR223585 representing NM_023066
  Red=Cloning site Green=Tags(s)
  
  MAPRKNAKGGGGNSSSSGSGSGSGSGSPSTGSSGSSSSPGARREAKHGGHKNGRRGGISGGSFFTWFMVI
  ALLGVWTSVAVVWFDLVDYEEVLGKLGVYDADGDGDFDVDDAKVLLGLKERSPSERTFPPEEEAETHAEL
  EEQAPEGADIQNVEDEVKEQIQSLLQESVHTDHDLEADGLAGEPQPEVEDFLTVTDSDDRFEDLEPGTVH
  EEIEDTYHVEDTASQNHPNDMEEMTNEQENSDPSEAVTDAGVLLPHAEEVRHQDYDEPVYEPSEHEGVAI
  SDNTIDDSSIISEEINVASVEEQQDTPPVKKKKPKLLNKFDKTIKAELDAAEKLRKRGKIEEAVNAFEEL
  VRKYPQSPRARYGKAQCEDDLAEKQRSNEVLRRAIETYQEAADLPDAPTDLVKLSLKRRSERQQFLGHMR
  GSLLTLQRLVQLFPSDTTLKNDLGVGYLLLGDNDSAKKVYEEVLNVTPNDGFAKVHYGFILKAQNKISES
  IPYLKEGIESGDPGTDDGRFYFHLGDAMQRVGNKEAYKWYELGHKRGHFASVWQRSLYNVNGLKAQPWWT
  PRETGYTELVKSLERNWKLIRDEGLMVMDKAKGLFLPEDENLREKGDWSQFTLWQQGRKNENACKGAPKT
  CALLEKFSETTGCRRGQIKYSIMHPGTHVWPHTGPTNCRLRMHLGLVIPKEGCKIRCANETRTWEEGKVL
  IFDDSFEHEVWQDASSFRLIFIVDVWHPELTPQQRRSLPAI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 83.5 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_075553
• Locus ID: 65973
• UniProt ID: Q8BSY0, Q6P8S1
• Cytogenetics: 4 A1
• Refseq Size: 6688
• Refseq ORF: 2223
• Synonyms: 2310005F16Rik; 3110001L23Rik; AI848629; AW261690; AW561901; BAH; C79816; cI-37
• Summary: Isoform 1: specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins.[UniProtKB/Swiss-Prot Function]