Tbk1 (NM_019786) Mouse Recombinant Protein
SKU
TP523008
Purified recombinant protein of Mouse TANK-binding kinase 1 (Tbk1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
$1,020.00
4 Weeks*
Specifications
| Product Data | |
| Species | Mouse |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-MYC/DDK |
| Predicted MW | 83.4 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C after receiving vials. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_062760 |
| Locus ID | 56480 |
| UniProt ID | Q9WUN2 |
| Cytogenetics | 10 D2 |
| RefSeq Size | 3031 |
| RefSeq ORF | 2187 |
| Synonyms | 1200008B05Rik; AI462036; AW048562 |
| Summary | Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents (PubMed:10581243, PubMed:15210742, PubMed:15661922). Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X (By similarity). This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB (By similarity). In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli (By similarity). Thus, several scaffolding molecules including FADD, TRADD, MAVS, AZI2, TANK or TBKBP1/SINTBAD can be recruited to the TBK1-containing-complexes (By similarity). Plays a key role in IRF3 activation: acts by first phosphorylating innate adapter proteins MAVS, TMEM173/STING and TICAM1 on their pLxIS motif, leading to recruitment of IRF3, thereby licensing IRF3 for phosphorylation by TBK1 (By similarity). Under particular conditions, functions as a NF-kappa-B effector by phosphorylating NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus (By similarity). Restricts bacterial proliferation by phosphorylating the autophagy receptor OPTN/Optineurin on 'Ser-177', thus enhancing LC3 binding affinity and antibacterial autophagy (By similarity). Phosphorylates SMCR8 component of the C9orf72-SMCR8 complex, promoting autophagosome maturation (By similarity). Phosphorylates and activates AKT1 (By similarity). Seems to play a role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity (PubMed:23396211).UniProtKB/Swiss-Prot Function |
| Protein Categories | Enzyme: Kinases, Growth Factors, Intracellular Proteins, Nervous system: Neurodegenerative diseases |
Reviews
Documents
| FAQs |
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Resources
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Citations
Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death
Sellier, Campanari, Julie Corbier et al
EMBO J. (2016)
Sellier, Campanari, Julie Corbier et al
EMBO J. (2016)
Cited by 414
AI Summary: OriGene contributed to this study by providing PCMV6 plasmids containing C-terminally Flag-tagged human cDNAs for SQSTM1 (P62), OPTN, SMCR8, WDR41, ULK1, NAP1, SINTBAD, TANK, and RAB GTPases, which were used to investigate the molecular mechanisms underlying autophagy regulation in ALS-FTD.
TRIM14 is a mitochondrial adaptor that facilitates retinoic acid-inducible gene-I-like receptor-mediated innate immune response
Zhou, Jia, Xue et al
Proc Natl Acad Sci U S A (2014) 111 (2), E245-54 DOI: 10.1073/pnas.1316941111
Zhou, Jia, Xue et al
Proc Natl Acad Sci U S A (2014) 111 (2), E245-54 DOI: 10.1073/pnas.1316941111
Cited by 137
AI Summary: In this paper, OriGene products—specifically, the TRIM14 expression plasmid (RC208706) and TRIM14 shRNA (TL312073)—were used to manipulate TRIM14 levels in cells, enabling the authors to investigate TRIM14’s role in the MAVS-mediated innate immune response.
Showing 1-2 of 2 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
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