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Kdm4a (NM_001161823) Mouse Recombinant Protein

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SKU
TP521564
Purified recombinant protein of Mouse lysine (K)-specific demethylase 4A (Kdm4a), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Size:
20 ug
  $1,020.00
4 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Mouse
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>MR221564 representing NM_001161823
Red=Cloning site Green=Tags(s)

MASESETLNPSARIMTFYPTMEEFRNFSRYIAYIESQGAHRAGLAKVVPPKEWKPRTSYDDIDDLVIPAP
IQQLVTGQSGLFTQYNIQKKAMTVREFRKIANSDKYCTPRYSEFEELERKYWKNLTFNPPIYGADVNGTL
YEQHVDEWNIGRLKTILDLVEKESGITIEGVNTPYLYFGMWKTSFAWHTEDMDLYSINYLHFGEPKSWYS
VPPEHGKRLERLAKGFFPGSAQSCEAFLRHKMTLISPLMLKKYGIPFDKVTQEAGEFMITFPYGYHAGFN
HGFNCAESTNFATRRWIEYGKQAVLCSCRKDMVKISMDVFVRRFQPERYKLWKAGKDSMVIDHTLPTPEA
AEFLKDSGGLTPRAGSEECPEEDVEAADQGEEGDVKRSLAKHRIGTKRHRVCLEIPQEVSQSELFPKEEL
SSGQYEMTECPATLAPVRPTHSSVRQVEDSLPFPDYSDPTEVKFEELKNVKLEEEDEEDEPEAAALDLSV
NPASVGGRLVFSGSKKKSSSSLGSTSSQDSVSSDSETAESVSCQGQEKTGVLTVHSYARGDGKAATGEPS
VKKKRSAPRSISEQELAEVADEYMLSLEENKKTKGRRQPLSKLPRHHPLVLQECGSDDETSEQLTPEEEA
EETEAWAKPLSQLWQNRPPNFEAEKEFNEIMAQQAPHCAVCMIFQTYHQVEFGAFSQSCGDASEPAAQTQ
RTKPLIPEMCFTTTGCSTDINLSTPYLEEDGTSMLVSCKKCSVRVHASCYGVPPAKASEEWMCSRCSANA
LEEDCCLCSLRGGALQRANDDRWVHVSCAVAILEARFVNIAERSPVDVSKIPLPRFKLKCVFCKKRRKRN
AGCCVQCSHGRCPTAFHVSCAQAAGVMMQPDDWPFVVFITCFRHKIPNLERAKGALLSITAGQKVISKHK
NGRFYQCEVVRLTTETFYEVNFDDGSFSDNLYPEDIVSQDCLQLGPPAEGEVVQVRWTDGQVYGAKFVAS
HPIQMYQVEFEDGSQLVVKRDDVYTLDEELPKRVKSRLSVASDMRFNEIFTEKEVKQEKKRQRVINSRYR
EDYIEPALYRAIME

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-MYC/DDK
Predicted MW 120.8 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_001155295
Locus ID 230674
UniProt ID Q8BW72
Cytogenetics 4 54.31 cM
RefSeq Size 4616
RefSeq ORF 3192
Synonyms D4Ertd222e; JHDM3A; Jmjd2; Jmjd2a; mKIAA0677
Summary Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code (PubMed:24953653). Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively (By similarity).UniProtKB/Swiss-Prot Function
Protein Categories Intracellular Proteins
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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