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Ube2o (NM_173755) Mouse Recombinant Protein

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SKU
TP520692
Purified recombinant protein of Mouse ubiquitin-conjugating enzyme E2O (Ube2o), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Size:
20 ug
  $1,020.00
4 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Mouse
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>MR220692 representing NM_173755
Red=Cloning site Green=Tags(s)

MADPAAPAPAQAQAAAAPTPAAAPAAAAPPPAPATDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVH
FGLVRLIHGEDSDSEGDDDGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDR
SVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYD
LKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPV
LSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSIITQENLGRVKRLGCFDHAQRQLGERCLY
VFPAKVEPAKIAWECPEKNCAQGEGSMAKKVKRLLKKQVVRIMSCTPDTQCPRDHSMEDPDKKGEARAGS
EIGSASPEEQPDGSASPVEMQDEGSEELQETCEPLPPFLLKEGGDDGLHSAEQDADDEAADDTDDTSSVT
SSASSTTSSQSGSGTGRKKSIPLSIKNLKRKHKRKKNKVTRDFKPGDRVAVEVVTTMTSADVMWQDGSVE
CNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIG
EEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGALPKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQ
HLYNIESEIEESDYDSVEGSSSGASSDEWEDDSDSWETDNGLVDDEHPKIEELAAILPAEQPTAPEEDKG
VVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVE
PEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAVPDTERKEEKPEVQSPVKAEWPSETPVLCQQC
GGRPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMD
LFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTE
RWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVF
EQEIRQHFSVGGWRLVNRIESWLETHAMQERAQVMPNGALKDSSSLEPMAAAELSDSGREEPEDVGMAPG
EASQGSDSEGGAQGPASASRDHTEQTETAPDASAPPSVRPKRRRKSYRSFLPEKSGYPDIGFPLFPLSKG
FIKSIRGVLTQFRAALLEAGMPESTEDK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-MYC/DDK
Predicted MW 141.3 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_776116
Locus ID 217342
UniProt ID Q6ZPJ3
Cytogenetics 11 E2
RefSeq Size 5192
RefSeq ORF 3864
Synonyms 9630022H21; B230113M03Rik; E2-230K; mKIAA1734
Summary E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins. Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity. Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis. Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location. Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly.UniProtKB/Swiss-Prot Function
Protein Categories Intracellular Proteins
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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