Aars2 (NM_198608) Mouse Recombinant Protein

CAT#: TP519930

Purified recombinant protein of Mouse alanyl-tRNA synthetase 2, mitochondrial (Aars2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR219930 representing NM_198608
  Red=Cloning site Green=Tags(s)
  
  MAVALAAAAGKLRRAIGRSCPWQPFSTEPGPPHGAAVRDAFLSFFRDRHGHRLVPSATVRPRGDPSLLFV
  NAGMNQFKPIFLGTVDPRSEMAGFRRVVNSQKCVRAGGRHNDLEDVGRDLSHHTFFEMLGNWAFGGEYFK
  EEACSMAWELLTQVYGIPEDRLWVSYFSGDSQTGLDPDLETRDIWLSLGVPASRVLSFGPQENFWEMGDT
  GPCGPCTEIHYDLAGGVGSPQLVELWNLVFMQHYREADGSLQLLPQRHVDTGMGLERLVAVLQGKRSTYD
  TDLFSPLLDAIHQSCGAPPYSGRVGAADEGRIDTAYRVVADHIRTLSVCIADGVSPGMSGAPLVLRRILR
  RAVRYSTEVLQAPPGFLGSLVPVVVETLGSAYPELEKNSVKIASLVSEDEAAFLASLQRGRRIIDRTVKR
  LGPSDLFPAEVAWSLSLSGNLGIPLDLVELMLEEKGVKLDTAGLEQLAQKEAQHRAQQAEADQEDRLCLD
  VHALEELHRQGIPTTDDSPKYNYTLHPNGDYEFGLCEARVLQLYSETGTAVASVGAGQRCGLLLDRTNFY
  AEQGGQASDRGYLVRTGQQDMLFPVAGAQLCGGFILHEAMAPERLQVGDQVQLYVDKAWRMGCMVKHTAT
  HLLSWALRQTLGPTTEQRGSHLNPERLRFDVATQTLLTTEQLRTVESYVQEVVGQDKPVFMEEVPLAHTA
  RIPGLRSLDEVYPDPVRVVSVGVPVAHALGPASQAAMHTSVELCCGTHLLSTGAVGDLVIIGERQLVKGI
  TRLLAITGEQAQQAREVGQSLSQEVEAASERLSQGSRDLPEAHRLSKDIGRLTEVAESAVIPQWQRQELQ
  TTLKMLQRRANTAIRKLEKGQATEKSQELLKRHSEGPLIVDTVSAESLSVLVKVVRQLCKQAPSISVLLL
  SPQPTGSVLCACQVAQDATPTFTAEAWALAVCSHMGGKAWGSRVVAQGTGHTADLEAALGTARAYALSQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 106.8 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_941010
• Locus ID: 224805
• UniProt ID: Q14CH7
• Cytogenetics: 17 B3
• Refseq Size: 3368
• Refseq ORF: 2940
• Synonyms: Aarsl; AlaRS; Gm89
• Summary: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.[UniProtKB/Swiss-Prot Function]