Gcfc2 (NM_177884) Mouse Recombinant Protein

SKU TP519015

Purified recombinant protein of Mouse GC-rich sequence DNA binding factor 2 (Gcfc2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR219015 representing NM_177884; Red=Cloning site Green=Tags(s); ; MALRPQRTFRRRQVESSDSDSDSDGAKEQSAEEPASAGGRTEGAERPRGARSARGRGRVWASSRRSPGAA; PRGDGGAECRTAELSTDEEEGTHTLTGSKGDRSPSSDSSCSLEERDVSPIVEIPDAAFIQAARRKRELAR; TPGDYISLDVNHSCSTSDCKRSNEEDPESDPDDHEKRILFTPKPQTLRQRMAEETSIRSEESSEESQEDE; NQDIWEQQQMRKAVRIPAGQNTDLSHSSKSQTLKKFDTSISFPPVNLEIIKKQLNNRLTLLQESHRSHQR; EYEKYEQDIKSSKTAIQNLESASDHAQNYRFYRGMKSYVENIIDCLNEKIVSIVELESSMYTLLLKRSEA; LLKRRQDELKCESSYLQQLSRKDETSANGSLAVDEKDQRILEEIEARRMQRRQARELSGSCDHQEGMSSD; DELSPAEMTNFHKCQGDILQDCKKVFEDVHDDFCNVQNILLKFQQWREKFPDSYYEAFVGFCLPKLLSPL; IRVQLLDWNPLKMDSIGLDKMPWFTAITEFMESSMDDIGKEDGSDKKILAAVINKTVVPRLTDFVETIWD; PLSTSQTRSLTVHCRVAFEQFASENEVSKNKQDLLKSIVARMKKSIEDDIFIPLYPKSSEEGKMSPHSKF; QERQFWGALKLFRNILLWNGLLPDDTLQDLGLGKLLNRYLIISLTNAVPGPDVVKKCSQIAACLPERWFE; NSAMRTSIPQLENFIKFLLQSAQKLSSSEFRNEVSEIILILVKVKALTQAESLREERPLEPLPAQSTGV; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 87.4 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_808552
• Locus ID: 330361
• UniProt ID: Q8BKT3
• Cytogenetics: 6 C3
• RefSeq Size: 3375
• RefSeq ORF: 2307
• Synonyms: A130099G21; AW146020; GCF2; Tcf9
• Summary: Factor that represses transcription. It binds to the GC-rich sequences (5'-GCGGGGC-3') present in the epidermal growth factor receptor, beta-actin, and calcium-dependent protease promoters. Involved in pre-mRNA splicing through regulating spliceosome C complex formation. May play a role during late-stage splicing events and turnover of excised inrons (By similarity).[UniProtKB/Swiss-Prot Function]