Sema3d (NM_028882) Mouse Recombinant Protein

CAT#: TP517350

Purified recombinant protein of Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (Sema3d), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR217350 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNVTKDENPRSRSQDLHLFHAWMMLIMTVLFLPVTETSKQNIPRLKLTYKDLLLSNTCIPFLGSSEGLDF
  QTLLLDEERGILLLGAKDHVFLLSLVDLNKNFKKIYWPAAKERVELCKLAGKDANAECANFIRVLQPYNK
  THVYVCGTGAFHPLCGYIDLGANKEELIFKLDTHNLESGRLKCPFDPQQPFASVMTDEHLYSGTASDFLG
  KDTAFTRSLGLMQDHHSIRTDISEHHWLNGAKFIGTFPIPDTYNPDDDKIYFFFRESSQEGSTSDRSILS
  RVGRVCKNDVGGQRSLINKWTTFLKARLICSIPGSDGADTHFDELQDIYLLPTRDERNPVVYGVFTTTSS
  IFKGSAVCVYSMADIRAVFNGPYAHKESADHRWVQYDGRIPYPRPGTCPSKTYDPLIKSTRDFPDDVISF
  IRRHPVMYKSVYPVAGAPTFKRINVDYRLTQIVVDHVVAEDGQYDVMFLGTDIGTVLKVVSISKEKWNME
  EVVLEELQVFKHPTAILNMELSLKQQQLYVGSWDGLVQLSLHRCDTYGKACADCCLARDPYCAWDGNACS
  RYAPTSKRRARRQDVKYGDPITQCWDIEDSISHETADEKVIFGIEFNSTFLECIPKSQQASVEWYIQRSG
  DEHREELKPDERIIKTDYGLLIRSLQKKDSGMYYCKAQEHTFIHTIVKLTLNVIENEQMENTQRAEYQEG
  QVKDLLAESRLRYKDYIQILSSPNFSLDQYCEQMWYKEKRRQRNKGSPKWKHMQEMKKKRNRRHHRDLDE
  LQRSVAT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 89.5 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_083158
• Locus ID: 108151
• UniProt ID: Q8BH34
• Cytogenetics: 5 A1
• Refseq Size: 6521
• Refseq ORF: 2334
• Synonyms: 4631426B19Rik
• Summary: Induces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Binds to neuropilin (By similarity).[UniProtKB/Swiss-Prot Function]