Alg1 (NM_145362) Mouse Recombinant Protein

CAT#: TP515774

Purified recombinant protein of Mouse asparagine-linked glycosylation 1 (beta-1,4-mannosyltransferase) (Alg1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR215774 representing NM_145362
  Red=Cloning site Green=Tags(s)
  
  MAASCVALLVLALLLLVLLLGLWKRGRQTGRARHMVVVVLGDVGRSPRMQYHALSLAQSGFSVTLLGFYN
  SKPRDELLQNDRIRIVKLTDLRGLGAGPRILQYGVKVVFQAVYLLWKMMRMDPAAYIFLQNPPGLPAIAV
  CWFVGCICGSKLVIDWHNYGYSIMGLVHGPRHPIVLLAKWYEKFFGRLSHLNLCVTNAMREDLAENWCVR
  AVTLYDKPASFFKETPLDLQHELFMKLSHTYSPFQSCSDPSHPDTERSAFTERDCQSGVVRRLHGRPALL
  VSSTSWTEDEDFSILLRALEKFEQQALTGDSLPSLVCVITGKGPLREHYRHLISQKHLQHVRFCTPWLEA
  EDYPLLLGSADLGVCLHMSSSGLDLPMKVVDMFGCHLPVCAVNFKCLHELVRHGENGLVFKDAEELAAQL
  QMLFSKFPDPAGKLSQFRKKLQESGQQRWDESWQHTVLPLLAHSQMTPRPHPPCGHPSCRGF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 54.9 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_663337
• Locus ID: 208211
• UniProt ID: Q921Q3
• Cytogenetics: 16 A1
• Refseq Size: 1772
• Refseq ORF: 1446
• Synonyms: HMAT1; HMT1
• Summary: Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man(5) intermediate on the cytoplasmic surface of the ER (By similarity).[UniProtKB/Swiss-Prot Function]