Gif (NM_008118) Mouse Recombinant Protein

SKU TP515639

Purified recombinant protein of Mouse cobalamin binding intrinsic factor (Cblif), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR215639 protein sequence; Red=Cloning site Green=Tags(s); ; MAWLTLYLLSVLWAVAGTSTRAQSSCSVPPDQQPWVDGLQALMENSVTDSDFPNPSILIAMNLAGAYNVE; AQKLLTYQLMASDSADLTSGQLALTVMALTSSCRDPGSKVSTLLKKMENWSPSSPGAESSAFYGPGLAIL; ALCQKSSEATLPIAVRFAKTLMMEPSPFNVDTGAVATLALTCMYNKIPVGSQENYRDLFGQALKAIVEKI; SLRIKADGIIGDIYSTGLAMQALSVTPEQPTKKWDCEKTMHTILNEIKQGKFQNPMSIAQILPSLKGKTY; LDVPQVTCGPDHEVPPTLTDYPTPVPTSVSNITVIYTINNQLRGVDLLFNVTIEVSVKSGSVLLAVLEEA; QRKNSMFKFETTMTSWGLIVSSINNIAENVNHKTYWEFLSGKTPLDEGVAYYIPFNHEHITANFTQY; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 45.5 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_032144
• Locus ID: 14603
• UniProt ID: P52787
• Cytogenetics: 19 A
• RefSeq Size: 1532
• RefSeq ORF: 1251
• Synonyms: AV073125
• Summary: Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the CBLIF-cobalamin complex is internalized via receptor-mediated endocytosis (By similarity).[UniProtKB/Swiss-Prot Function]