Mtor (NM_020009) Mouse Recombinant Protein
SKU
TP512124
Purified recombinant protein of Mouse mechanistic target of rapamycin kinase (Mtor), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
$1,020.00
4 Weeks*
Specifications
| Product Data | |
| Species | Mouse |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-MYC/DDK |
| Predicted MW | 289.2 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C after receiving vials. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_064393 |
| Locus ID | 56717 |
| UniProt ID | Q9JLN9 |
| Cytogenetics | 4 78.76 cM |
| RefSeq Size | 8612 |
| RefSeq ORF | 7647 |
| Synonyms | 2610315D21Rik; AI327068; flat; FRAP; Frap1; FRAP2; RAFT1; RAPT1 |
| Summary | Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals (PubMed:15467718, PubMed:15545625, PubMed:16221682, PubMed:16915281, PubMed:16962653, PubMed:18046414, PubMed:19440205, PubMed:21659604). MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins (PubMed:15467718, PubMed:15545625, PubMed:16221682, PubMed:16915281, PubMed:16962653, PubMed:18046414, PubMed:19440205, PubMed:21659604). Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2) (PubMed:15467718, PubMed:16962653, PubMed:21659604). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis (By similarity). This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E) (By similarity). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4 (By similarity). Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex (By similarity). Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor (By similarity). In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1 (PubMed:11792863). To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A (PubMed:18046414). mTORC1 also negatively regulates autophagy through phosphorylation of ULK1 (PubMed:21258367). Under nutrient sufficiency, phosphorylates ULK1 at 'Ser-758', disrupting the interaction with AMPK and preventing activation of ULK1 (PubMed:21258367). Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP (By similarity). Also prevents autophagy by phosphorylating RUBCNL/Pacer under nutrient-rich conditions (By similarity). mTORC1 exerts a feedback control on upstream growth factor signaling that includes phosphorylation and activation of GRB10 a INSR-dependent signaling suppressor (PubMed:21659604). Among other potential targets mTORC1 may phosphorylate CLIP1 and regulate microtubules (By similarity). As part of the mTORC2 complex MTOR may regulate other cellular processes including survival and organization of the cytoskeleton (By similarity). Plays a critical role in the phosphorylation at 'Ser-473' of AKT1, a pro-survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1 (By similarity). mTORC2 may regulate the actin cytoskeleton, through phosphorylation of PRKCA, PXN and activation of the Rho-type guanine nucleotide exchange factors RHOA and RAC1A or RAC1B (By similarity). mTORC2 also regulates the phosphorylation of SGK1 at 'Ser-422' (By similarity). Regulates osteoclastogenesis by adjusting the expression of CEBPB isoforms (PubMed:19440205). Plays an important regulatory role in the circadian clock function; regulates period length and rhythm amplitude of the suprachiasmatic nucleus (SCN) and liver clocks (PubMed:29750810).UniProtKB/Swiss-Prot Function |
| Protein Categories | Enzyme: Kinases, Growth Factors, Intracellular Proteins |
Reviews
Documents
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Resources
| Recombinant Protein Resources |
Citations
The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2
Kovalski, Bhaduri, Zehnder et al
Mol. Cell (2019) 73 (4), 830-844.e12 DOI: 10.1016/j.molcel.2018.12.001
Kovalski, Bhaduri, Zehnder et al
Mol. Cell (2019) 73 (4), 830-844.e12 DOI: 10.1016/j.molcel.2018.12.001
AI Summary: In this study, OriGene’s recombinant proteins—including NRASQ61K (Cat# TP701004), NRAS (Cat# TP302681), MAPKAP1 (Cat# TP311745), mTOR (Cat# TP320457), KRASG12D (Cat# TP700052), HRASWT (Cat# TP316409), and custom-produced HRASG12V—were used to investigate and validate the interactions between oncogenic Ras proteins and mTORC2 components, elucidating key mechanisms in Ras-driven cancer proliferation.
Snippet: … They demonstrate that active Ras binds and stimulates mTORC2, representing a key dependency for Ras-driven proliferation genes and tumorigenesis. Cancer Ras Proteomics CRISPR mTOR MAPKAP1;... … They demonstrate that active Ras binds and stimulates mTORC2, representing a key dependency for Ras-driven proliferation genes and tumorigenesis. Cancer Ras Proteomics CRISPR mTOR MAPKAP1;NRASQ61K Human Recombinant Protein Origene Cat# TP701004 NRAS Human Recombinant Protein Origene Cat# TP302681 MAPKAP1 Human Recombinant Protein Origene Cat# TP311745 mTOR Human Recombinant Protein Origene Cat# TP320457 KRASG12D Human Recombinant Protein Origene Cat# TP700052 HRASWT Human Recombinant Protein Origene Cat# TP316409 HRASG12V Human Recombinant Protein Origene Custom Production from clone Cat# RC400129
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