Uba1 (NM_001136085) Mouse Recombinant Protein

SKU
TP511556
Purified recombinant protein of Mouse ubiquitin-like modifier activating enzyme 1 (Uba1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
  $1,020.00
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Specifications
Specifications
Product Data
Species Mouse
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>MR211556 protein sequence
Red=Cloning site Green=Tags(s)

MSSSPLSKKRRVSGPDPKPGSNCSPAQSALSEVSSVPTNGMAKNGSEADIDESLYSRQLYVLGHEAMKML
QTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTTQWADLSSQFYLREEDIGKNRAEVSQPRLAELNS
YVPVTAYTGPLVEDFLSSFQVVVLTNSPLEAQLRVGEFCHSRGIKLVVADTRGLFGQLFCDFGEEMVLTD
SNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFETGDFVSFSEVQGMIQLNGCQPMEIKVLGPYTFSICDT
SNFSDYIRGGIVSQVKVPKKISFKSLPASLVEPDFVMTDFAKYSRPAQLHIGFQALHQFCALHNQPPRPR
NEEDATELVGLAQAVNARSPPSVKQNSLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPI
MQWLYFDALECLPEDKEALTEEKCLPRQNRYDGQVAVFGSDFQEKLSKQKYFLVGAGAIGCELLKNFAMI
GLGCGEGGEVVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPYIQVTSHQNRVGPDTERIY
DDDFFQNLDGVANALDNIDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIP
ICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDSKFVERTLRLAGTQPLEVLEAVQRSLVLQRP
QTWGDCVTWACHHWHTQYCNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNTLHLDYVMAAAN
LFAQTYGLTGSQDRAAVASLLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLP
GFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDISPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELY
KVVQGHQQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFK
TEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEV
PYVRYTIR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-MYC/DDK
Predicted MW 117.8 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_001129557
Locus ID 22201
UniProt ID Q02053
Cytogenetics X 16.15 cM
RefSeq Size 4073
RefSeq ORF 3174
Synonyms A1; A1S9; Sb; Sbx; Ube-1; Ube1x
Summary This gene encodes a member of the ubiquitin-activating E1 family. The encoded protein initiates the ubiquitin activation and transfer cascade, catalyzing the first step in ubiquitin conjugation to mark cellular proteins for proteasome degradation. Ubiquitin activating enzymes use ATP to form a thioester between a conserved catalytic cysteine of the enzyme and the C-terminal carboxylate of ubiquitin. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jan 2013
Protein Categories Enzyme: Ligase, Intracellular Proteins, Membrane Proteins, Nervous system: Neurodegenerative diseases
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