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Smg8 (NM_024262) Mouse Recombinant Protein

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SKU
TP511398
Purified recombinant protein of Mouse smg-8 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg8), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Size:
20 ug
  $1,020.00
4 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Mouse
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>MR211398 protein sequence
Red=Cloning site Green=Tags(s)

MAGPVSLRELLMGASAWLGSESPGGPSAEGGGNAAGTPEPPWREDEICVVGIFGKTALRLNSEKFSLVNT
VCDRQVFPLFHHQDPGDPGTGIKTKAVSVGEAGGAGDPGAAAGDSLRGGMATAEGNRAEPGPQDFSLLQA
YYNQESKVLYLILTSICDNSQLLRACRALQSGEAGGGLSLPHAETHEFWKHQEKLQCLSLLYLFSVCHIL
LLVHPTCSFDITYDRVFRALDGLRQKVLPLLKTAIKDCPVGKDWKLNCRPCPPRLLFLFQLNGALKVEPP
RSQDTAHPDKPKKHSPKRRLQHALEDQIYRIFRKSRVLTNQSINCLFTVPANQAFVYIVPGSQEEDPIGM
LLDQLRSHCTVKDPESLLVPAPLSGPRRYQAMRQHSRQQLSFHIDSSTSSSSGQLVDFTLREFLWQHVEL
VLSKKGFDDSVGRNPQPSHFELPTYQKWISAAAKLYEVAIDGKEEDLGSPTGELTSKILSSIKVLEGFLD
IDTKFSENRCQKALPMAHSAYQSNLPHNYTMTVHKNQLAQALRVYSQHARGPAFHKYAMQLHEDCYKFWS
NGHQLCEERSLTDQHCVHKFHSLPKSGEKPEADRNPPVLYHNSRARSTGACNCGRKQAPRDDPFDIKAAN
YDFYQLLEEKCCGKLDHINFPVFEPSTPDPAPAKNEPSPAPPDSDAEKLKEKEPQTQGESTSLSLALSLG
QSTDSLGTYPADPQAGGDNPEVHGQGEGKSEKRPNLVDRQASTVEYLPGMLHSNCPKGLLPKFSSWSLVK
LGPAKSYNFHTGLDQQGFVPGTNYLMPWDIVIRTRAEDEGDLDTNSWPAPNKAIPGKRSAVVMGRGRRRD
DIARAFVGFEYEDSRGRRFMCSGPDKVMKVMGSGPKESALKALNSDMPLYILSSSQGRGLKPHYAQLMRL
FVVVPDAPLQIILMPQVQPGPPPCPVFYPEKQEITLPPDGLWVLRFPYAYVTERGPCFPPKENVQLMSYK
VLRGVLKAVTQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-MYC/DDK
Predicted MW 109.7 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_077224
Locus ID 74133
UniProt ID Q8VE18
Cytogenetics 11 C
RefSeq Size 3236
RefSeq ORF 2973
Synonyms 1200011M11Rik
Summary Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited by release factors to stalled ribosomes together with SMG1 and SMG9 (forming the SMG1C protein kinase complex) and, in the SMG1C complex, is required to mediate the recruitment of SMG1 to the ribosome:SURF complex and to suppress SMG1 kinase activity until the ribosome:SURF complex locates the exon junction complex (EJC). Acts as a regulator of kinase activity (By similarity).UniProtKB/Swiss-Prot Function
Protein Categories Intracellular Proteins, Membrane Proteins
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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