Arhgef1 (NM_001130152) Mouse Recombinant Protein

CAT#: TP511176

Purified recombinant protein of Mouse Rho guanine nucleotide exchange factor (GEF) 1 (Arhgef1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR211176 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGEVAGGAAPGPPRSGLVSIIIGAEDEDFENELEANSEDQNSQFQSLEQVKRRPAHLMALLQHVALQFEP
  GPLLCCLHADMLSSLGPKEAKKAFLDFYHSFLEKTAVLRVPVPPSVAFELDRTRPDLISEDVQRRFIQEV
  VQSQQAAVSRQLEDFRSKRLMGMTPWEQELSLLEPWIGKDRGNYEARERHVAERLLSHLEETQHTISTDE
  EKSAAVVTAISLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPPKTKKGLSSILDPARWNRGEPSAP
  DCRHLKVEADAEKPGPADRKGGLGMSSRDRTVGTPGQDNPGVSLHPLSTDSVDSREPGVDTPQEPGDTPP
  QGPTSLEPLAPPESTEDNGETESPEPGDDGEPGRSGLELEPEEPPGWRELVPPDTLLSLPKSQVKRQEVI
  SELLVTEAAHVRMLRVLHDLFYQPMADGGFFPLDELQNIFPSLDELIEVHSLFLDRLMKRRQESGYLIEE
  IGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCAFVQEAESRPRCRRLQLKDMIPTE
  MQRLTKYPLLLQSIGQNTEESTERGKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLTHLRQSS
  DPMLSEFKNLDITKKKLVHEGPLTWRVTKDKAIEVHVLLLDDLLLLLQRQDERLLLKSHSRTLTPTPDGK
  TMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTSSERKNWCNLITETAGSLKVPAPASRL
  KPRPSPSSIREPLLSSSENGTGGAEMAPADARTERLLNDLLPFCRPGPEGQLAATALQKVLSLKQILLST
  EEDSGAGPPRDGDGVPGGRAPGPVHTQEIEENLLSLEVAIRQLEELEEEFCRLRPLLSQLGGTLSPNLAA
  PERSAQTGLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 102.8 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_001123624
• Locus ID: 16801
• UniProt ID: Q61210
• Cytogenetics: 7 A3
• Refseq Size: 3282
• Refseq ORF: 2763
• Synonyms: Lbcl2; Lsc
• Summary: Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation. Isoform 3 and isoform 4 do not homooligomerize and show an enhanced RhoGEF activity.[UniProtKB/Swiss-Prot Function]