Gba2 (NM_172692) Mouse Recombinant Protein

CAT#: TP511175

Purified recombinant protein of Mouse glucosidase beta 2 (Gba2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR211175 representing NM_172692
  Red=Cloning site Green=Tags(s)
  
  MVTCVPASEQVGCAERDSQVYCEDTGGTEAVRVTDCGSPEDSGPQDEPSYCNSEDSGQLMASYEGKARGY
  QVPPFGWRICLAHEFAEKRRPFQANNISLSNLVKHLGMGLRYLKWWYRKTHVEKKTPFIDMLNSLPLRQI
  YGCPLGGIGGGTITRGWRGQFCRWQLNPGMYQHQTVIADQFIVCLRRDGRTVYQQVLSLELPNVLRSWNW
  GLCGYFAFYHALYPRAWTVYQLPGQNVTLTCRQVTPILPHDYQDSSLPVGVFVWDVENEGDETLDVSITF
  SMRNGLGGEDDAAGSLWNEPFRLEQGGTTVQGLLLHHPTPPNPYTMAVAARCTADTTVTHTTAFDPNGTG
  QQVWQDLLQDGQLDSPAGQSTPTQKGEGIAGAVCVSSKLLPRSRCCLEFSLAWDMPKIMFGAKSQVHYRR
  YTRFFGSDGDVAPALSHYALCHYADWEDRISAWQNPVLDDRTLPAWYKSALFNELYFLADGGTVWLEVPA
  DSLPEGLGGSMRQLRSTLQDYGRFGYLEGQEYRMYNTYDVHFYASFALVMLWPKLELSLQYDMALATLKE
  DLTRRRYLMSGVVAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKDLNLKFVLQIYRDYYLTGDQG
  FLEDMWPVCLAVMESEMKFDKDQDGLIENGGYADQTYDAWVTTGPSAYCGGLWLAAVAVMVQMAVLCGAQ
  DVQERFASILCRGREAYERLLWNGRYYNYDSSSHPQSRSIMSDQCAGQWFLRACGLGEGDTEVFPTLHVV
  RALQTIFELNVQAFAGGAMGAVNGMHPHGVPDRSSVQSDEVWVGVVYGLAATMIQEGLTWEGFRTAEGCY
  RTVWERLGLAFQTPEAYCQQQVFRSLAYMRPLSIWAMQLALQQQQHKKSRRPSVTQGTGLSTQPECGPKR
  SLANLNSE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 103.7 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_766280
• Locus ID: 230101
• UniProt ID: Q69ZF3
• Cytogenetics: 4 A5
• Refseq Size: 3552
• Refseq ORF: 2754
• Synonyms: F630034E04
• Summary: Non-lysosomal glucosylceramidase that catalyzes the hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide (PubMed:17080196, PubMed:23250757). Glucosylceramides are membrane glycosphingolipids that have a wide intracellular distribution (PubMed:23250757). They are the main precursors of more complex glycosphingolipids that play a role in cellular growth, differentiation, adhesion, signaling, cytoskeletal dynamics and membrane properties (PubMed:25803043). Also involved in the transglucosylation of cholesterol, transferring glucose from glucosylceramides, thereby modifying its water solubility and biological properties (PubMed:26724485). Under specific conditions, may catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide (PubMed:26724485). Finally, may also play a role in the metabolism of bile acids (PubMed:17080196). It is able to hydrolyze bile acid 3-O-glucosides but also to produce bile acid-glucose conjugates thanks to a bile acid glucosyl transferase activity (PubMed:17080196). However, the relevance of both activities is unclear in vivo (PubMed:17080196).[UniProtKB/Swiss-Prot Function]