Fig4 (NM_133999) Mouse Recombinant Protein

CAT#: TP511140

Purified recombinant protein of Mouse FIG4 phosphoinositide 5-phosphatase (Fig4), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR211140 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPTAAAPIISSVQKLVLYETRARYFLVGSNHAETKYRVLKIDRTEPKDLVVIDDRHVYTQQEVRELLGRL
  DLGNRTKMSQKGSSGLFRAVSAFGVVGFVRFLEGYYIVLITKRRKMADIGGHAIYKIEDTSMIYIPNDSV
  RISHPDEARYLRIFQNVDLSSNFYFSYSYDLSHSLQYNLTVLRMPLEMLKSETSKACQESFDIFEDEGLI
  TQGGSGVFGISSEPYMKYVWNGELLDIIKNTVHRDWLLYIIHGFCGQSKLLIYGRPVYVTLIARRSSRFA
  GTRFLKRGANCEGDVANEVETEQILCDASVMSFTAGSYSSYVQVRGSVPLFWSQDISTMMPKPPITLDQA
  DPFAHVAALHFDQMLQRFGSPIIILNLVKEREKRKHERILSEELVAAVTYLNQFLPPEHTIVYIPWDMAK
  YTKSKLCNVLDRLNVIAESVVKKTGFFVNRPDSYCSILRPDEKWNELGGHVIPTGRLQTGILRTNCVDCL
  DRTNTAQFMVGKCALAYQLYSLGLIDKPNLQFDTDAVRLFEELYEDHGDTLSLQYGGSQLVHRVKTYRKI
  APWTQHSKDIMQTLSRYYSNAFSDADRQDSINLFLGVFHPTEGKPHLWELPTDFYLHHKNTMSLLPPRRS
  YTYWWTPEVVKHLPLPYDEVICAANLKKLMVKKFHRWEEEIDIHNEFFRPYELSSFDDTFCLAMTSSARD
  FMPKTVGIDPSPFTVRKPDETGKSVLGNKNTREEAVLQRKTAASAPPPPSEEAVSSSSEDDSGTDREDEG
  SISQRSTPVKMTDTGDSAKATENVVQPMKEVYGVSLSSSLSEEDHSIYARFVQLGQSQHKQDRGNQQLCS
  RCSDGVIKLTPISAFSQDNIYEVQPPRVDRKSTEIFQAHIQASQGIMQPLGKEDTAMYREYIRNRYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 103.4 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_598760
• Locus ID: 103199
• UniProt ID: Q91WF7
• Cytogenetics: 10 B1
• Refseq Size: 3278
• Refseq ORF: 2724
• Synonyms: A530089I17Rik; AI326867; Sac3
• Summary: The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). In vitro, hydrolyzes all three D5-phosphorylated polyphosphoinositide substrates in the order PtdIns(4,5)P2 > PtdIns(3,5)P2 > PtdIns(3,4,5)P3. Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.[UniProtKB/Swiss-Prot Function]