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Limch1 (BC075634) Mouse Recombinant Protein

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SKU
TP511106
Purified recombinant protein of Mouse LIM and calponin homology domains 1 (cDNA clone MGC:100029 IMAGE:30544497), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Size:
20 ug
  $1,020.00
4 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Mouse
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>MR211106 protein sequence
Red=Cloning site Green=Tags(s)

MHKTCTIRSFIRDFYRILFTCARPSIMSDDAESTSMFDMRCEEEAAVLPHSRARQEQLQPEPPPEPAFAE
AQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNTILFLRGCKELGLKE
SQLFDPSDLQDTSNRVTVKNLDYSRKLKNVLVTIYWLGKAANSCASYGGTTLNLKEFEGLLAQMRKETDD
IDSPKRSIRDSGYIDCWDSERSDSLSPPRHGREDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQ
EEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNVSSFPNDPSPMKYLRQQSLPPPKFTATVETT
IARTSVPESIASAGTGSPSKIITPNTVPMLTPRPYSQPKNSQEVLKTFKVDGKVSMNGEMAPGDEEGKEK
EGPAAAAPGPSLTKSQMFEGVATVHDSPVQVKQGSNSIEINIKKPNSAPQELTAASEETESNGQEDEDGE
ERPGTGDLEPDSAEPQHFTTTVTRCSPTVALVEFSPNPQLKNEVPEQGQKKPEDEMSGKVELVLSQKVAK
PKSPEPEATLTFPFLDKMPETNQLHLPNPSSQADSPSSEKSPGSTPFKFWAWDPEEERRRQEKWQQEQER
LLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTISSSSADQLSTSLSVTEGSGTRN
KMDLENCPDKENERRQKTPFQENDGDSLLKTREGGLPEEQSLTPSPSANPEISVSKGIHQDPQLEAEAGA
PHCGTNPQPAQDPPRNQQIPNPPTSTSEDVKPKTLALEKTINHQMESPGERRKSISGKKLCSSCGLTLGK
GAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCTDCYMRSRSAGQPTTL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-MYC/DDK
Predicted MW 100.7 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
Locus ID 77569
UniProt ID Q3UH68
Cytogenetics 5 C3.1
RefSeq Size 4698
RefSeq ORF 2703
Synonyms mKIAA1102
Summary Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.UniProtKB/Swiss-Prot Function
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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