Mastl (NM_025979) Mouse Recombinant Protein

CAT#: TP511003

Purified recombinant protein of Mouse microtubule associated serine/threonine kinase-like (Mastl), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR211003 representing NM_025979
  Red=Cloning site Green=Tags(s)
  
  MESASASEENEGGAAIEECVSRIPVPRPPSIEEFTIVKPISRGAFGKVYLGQKGGKLYAVKVVKKADMIN
  KNMTHQVQAERDALALSKSPFVVHLYYSLQSASNIYLIMEYLIGGDVKSLLHIYGYFDEEMAIKYISEVA
  LALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTLNRDINMMDILTTPSMSKPKQDYSRTPGQV
  LSLISSLGFFTPVGEKDQDSANMFSAPKSAAQLSRGFICPMSVDQKEPTSYSSKLLKSCFETLSSNPEIP
  VKCLTSNLLQCRKRLGTSSTSSQSHTFVSSVESECHSNPKWERDCQSTESSGCAMSWNAVEMLYAKSTSA
  IKTKTELELALSPIHDSSAIPAAGSNQVTLPRKCFREISWEARDPDNENMTIDKGQSGFCQSSQRSVNSS
  ATSEEHLGKRNYKRNFHLVDSSPCQEIMQSKKNCTEYEANKERQGCRANQSTGLTTEVQNLKLSGCESQQ
  LDYANKENIVTYLTDRQTPEKLHIPTIAKNLMSELDEDCELSSKKDCLSSNSVCSDEDRALKTTCVDSDS
  SFPGVSMMESSLEIQALEPDKSIRDYSFEEPNTEDLFVLPKCQENSLPQDDCHACIQDSSQVSAHPSKAP
  KALTSKINVVAFRSFNSHINASTNSEPSKISITSLDAMDISCDYSGSYPMAVSPTEKGRHYTSHQTPNQV
  KLGTSYRTPKSVRRGAAPVDDGRILGTPDYLAPELLLGTAHGPAVDWWALGVCLFEFLTGIPPFNDETPQ
  QVFQNILKRDIPWPEGEEKLSDNAQSAMDMLLTIDDSKRAGMRELKQHPLFSEVDWENLQHQTMPFVPQP
  DDETDTSYFEARNNAQHLTISGFSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 96.4 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_080255
• Locus ID: 67121
• UniProt ID: Q8C0P0
• Cytogenetics: 2 A3
• Refseq Size: 4652
• Refseq ORF: 2595
• Synonyms: 2700091H24Rik; C88295; GW; GWL; MAST-L; THC2
• Summary: Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited (By similarity).[UniProtKB/Swiss-Prot Function]