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Mastl (NM_025979) Mouse Recombinant Protein

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SKU
TP511003
Purified recombinant protein of Mouse microtubule associated serine/threonine kinase-like (Mastl), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Size:
20 ug
  $1,020.00
4 Weeks*
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Specifications
Specifications
Product Data
Species Mouse
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>MR211003 representing NM_025979
Red=Cloning site Green=Tags(s)

MESASASEENEGGAAIEECVSRIPVPRPPSIEEFTIVKPISRGAFGKVYLGQKGGKLYAVKVVKKADMIN
KNMTHQVQAERDALALSKSPFVVHLYYSLQSASNIYLIMEYLIGGDVKSLLHIYGYFDEEMAIKYISEVA
LALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTLNRDINMMDILTTPSMSKPKQDYSRTPGQV
LSLISSLGFFTPVGEKDQDSANMFSAPKSAAQLSRGFICPMSVDQKEPTSYSSKLLKSCFETLSSNPEIP
VKCLTSNLLQCRKRLGTSSTSSQSHTFVSSVESECHSNPKWERDCQSTESSGCAMSWNAVEMLYAKSTSA
IKTKTELELALSPIHDSSAIPAAGSNQVTLPRKCFREISWEARDPDNENMTIDKGQSGFCQSSQRSVNSS
ATSEEHLGKRNYKRNFHLVDSSPCQEIMQSKKNCTEYEANKERQGCRANQSTGLTTEVQNLKLSGCESQQ
LDYANKENIVTYLTDRQTPEKLHIPTIAKNLMSELDEDCELSSKKDCLSSNSVCSDEDRALKTTCVDSDS
SFPGVSMMESSLEIQALEPDKSIRDYSFEEPNTEDLFVLPKCQENSLPQDDCHACIQDSSQVSAHPSKAP
KALTSKINVVAFRSFNSHINASTNSEPSKISITSLDAMDISCDYSGSYPMAVSPTEKGRHYTSHQTPNQV
KLGTSYRTPKSVRRGAAPVDDGRILGTPDYLAPELLLGTAHGPAVDWWALGVCLFEFLTGIPPFNDETPQ
QVFQNILKRDIPWPEGEEKLSDNAQSAMDMLLTIDDSKRAGMRELKQHPLFSEVDWENLQHQTMPFVPQP
DDETDTSYFEARNNAQHLTISGFSL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-MYC/DDK
Predicted MW 96.4 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_080255
Locus ID 67121
UniProt ID Q8C0P0
Cytogenetics 2 A3
RefSeq Size 4652
RefSeq ORF 2595
Synonyms 2700091H24Rik; C88295; GW; GWL; MAST-L; THC2
Summary Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited (By similarity).UniProtKB/Swiss-Prot Function
Protein Categories Enzyme: Kinases, Intracellular Proteins
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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