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Usp16 (NM_024258) Mouse Recombinant Protein

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SKU
TP510833
Purified recombinant protein of Mouse ubiquitin specific peptidase 16 (Usp16), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Size:
20 ug
  $1,020.00
4 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Mouse
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>MR210833 protein sequence
Red=Cloning site Green=Tags(s)

MGKKRTKGRSAPDTVASESAEPVCRHLRKGLEQGNLKKALVNVEWNICQDCKTDNKVKDKPEEEAEDPSV
WLCLKCGHQGCGRDSQEQHALKHYTTPRSEPHYLVLSLDNWSVWCYKCDEEVKYCSSNRLGQVVDYVRKQ
AGVRTSKPAEKNNGHIELENKKLEKESKNEQEKEKSENLAKETIPMDSASQITVKGLSNLGNTCFFNAVM
QNLSQTPVLRELLKEVKMSGTIVKIEPPDLALTEPLEVNLEPPGPLTLAMSQFLSEMQENKKRVVTPKEL
FSQVCKKATRFKGYQQQDSQELLRYLLDGMRAEEHQRVSKGILKAFGNSTEKLDEEVKNKVKDYEKKKAI
PSFVDRIFGGELTSTIMCDECRTVSLVHESFLDLSLPVLDDQSGKKSINDKNVKMTMEEEDKDSEEEKDD
SYMKSRSDLPSGTSKHLQKKAKKQAKKQAKNQRRQQKIQERFLHFNELCATDYTEDNEREADTALAGEVE
VDTDSTHGSQEEATQIELSVNQKDLDGQESMIERTPDVQESPEDLGVKSANTESDLGIVTPAPECPRDFN
GAFLEERTSGELDIINGLKNLNLNAAVDPDEINIEIPNDSHSAPKVYEVMNEDPETAFCTLANREAFSTD
ECSIQHCLYQFTRNEKLQDANKLLCEVCSRRQCNGPKANIKGDRRHVYTNAKKQMLVSLAPPVLTLHLKR
FQQAGFNLRKVNKHIKFPEILDLAPFCTLKCKNVAEESTRVLYSLYGVVEHSGTMRSGHYTAYAKERTAS
CHLSNLVLHGDIPQDCEMESTKGQWFHISDTHVQAVPITKVLNSQAYLLFYERIL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-MYC/DDK
Predicted MW 93.4 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_077220
Locus ID 74112
UniProt ID Q99LG0
Cytogenetics 16 C3.3
RefSeq Size 2926
RefSeq ORF 2475
Synonyms 1200004E02Rik; 2810483I07Rik; 6330514E22Rik; UBPM
Summary Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes (PubMed:24034696). Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.UniProtKB/Swiss-Prot Function
Protein Categories Enzyme: Peptidases, Intracellular Proteins
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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