Sec23a (NM_009147) Mouse Recombinant Protein

CAT#: TP510540

Purified recombinant protein of Mouse SEC23 homolog A, COPII coat complex component (Sec23a), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR210540 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTTYLEFIQQNEERDGVRFSWNVWPSSRLEATRMVVPVAALFTPLKERPDLPPIQYEPVLCSRTTCRAVL
  NPLCQVDYRAKLWACNFCYQRNQFPPTYAGISELNQPAELLPQFSSIEYVVLRGPQMPLIFLYVVDTCIE
  DEDLQALKESMQMSLSLLPPTALVGLITFGRMVQVHELGCEGISKSYVFRGTKDLSAKQLQEMLGLSKVP
  VTQATRGPQVQQPPPSNRFLQPVQKIDMNLTDLLGELQRDPWPVPQGKRPLRSSGVALSIAVGLLECTFP
  NTGARIMMFIGGPATQGPGMVVGDELKTPIRSWHDIEKDNAKYVKKGTKHFEALANRAATTGHVIDIYAC
  ALDQTGLLEMKCCPNLTGGYMVMGDSFNTSLFKQTFQRVFTKDIHGQFKMGFGGTLEIKTSREIKISGAI
  GPCVSLNSKGPCVSENEIGTGGTCQWKICGLSPTTTLAIYFEVVNQHNAPIPQGGRGAVQFVTQYQHSSG
  QRRIRVTTIARNWADAQTQIQNIAASFDQEAAAILMARLAIYRAETEEGPDVLRWLDRQLIRLCQKFGEY
  HKDDPNSFRFSETFSLYPQFMFHLRRSPFLQVFNNSPDESSYYRHHFMRQDLTQSLIMIQPILYAYSFSG
  PPEPVLLDSSSILADRILLMDTFFQILIYHGETIAQWRKSGYQDMPEYENFRHLLQAPVDDAQEILHSRF
  PMPRYIDTEHGGSQARFLLSKVNPSQTHNNMYAWGQESGAPILTDDVSLQVFMDHLKKLAVSSAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 86.2 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_033173
• Locus ID: 20334
• UniProt ID: Q01405, Q8C1E4
• Cytogenetics: 12 C1
• Refseq Size: 4108
• Refseq ORF: 2298
• Synonyms: Msec23; Sec23r
• Summary: Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex (By similarity). Required for the translocation of insulin-induced glucose transporter SLC2A4/GLUT4 to the cell membrane (PubMed:27354378).[UniProtKB/Swiss-Prot Function]