Pcif1 (NM_146129) Mouse Recombinant Protein
CAT#: TP510164
Purified recombinant protein of Mouse PDX1 C-terminal inhibiting factor 1 (Pcif1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
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Specifications
Product Data | |
Species | Mouse |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence |
>MR210164 protein sequence
Red=Cloning site Green=Tags(s) MANENHGSPREGASLLSHSPGTSSQSQPCSPKPVRLVQDLPEELVHAGWEKCWSRRESRPYYFNRFTNQS LWEMPVLGQHDVLSDPLGLNATPLPQDSSLVETPPVENKSRKRQLSEEQPSGNGVKKPKIEIPVTPTSQS VPSSPSIPGTPTLKIWGSSTEDKQAALLRPTEVYWDLDIQTNAVIKHRGPSEVLPPHPDVELLRSQLILK LRQHYRELCQQREGIEPPRESFNRWMLERKVVDKGCDPLLPSNCEPVVSPSMFREIMNDIPIRLSRIKFR EEAKRLLFKYAEAARRLIESRSASPDSRKVVKWNVEDTFSWLRKEHSASKEDYMDRLEHLRRQCGPHVSA AAKDSVEGICSKIYHISLEYVKRIREKHLAVLKENNIPEEVEASELEPRLVYCYPVRLAVSAPPMPSVEM HVENSVVCIRYKGEMVKVSRSYFSKLWLLYRYSCVDDSAFERFLPRVWCLLRRYQMMFGVGLYEGTGLQG SLPVHVFETLHRLFGVSFECFASPLNCYFRQYCSAFPDTDGYFGSRGPCLDFTPLSGSFEANPPFCEELM DAMVSHFEKLLESSAEPLSFIVFIPEWREPPTPALTRMEQSRFKRHQLVLPAFEHEYRSGSQHICKKEEM HYKAVHNTAVLFLQNGPGFAKWGPTPERLQELTAAYKQSGRSHGSSSSSSSSSSSSEAKDRDSGREQGPS REPHPT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Tag | C-MYC/DDK |
Predicted MW | 80.5 kDa |
Concentration | >0.05 µg/µL as determined by microplate BCA method |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
Storage | Store at -80°C after receiving vials. |
Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Reference Data | |
RefSeq | NP_666241 |
Locus ID | 228866 |
UniProt ID | P59114, Q542C3 |
Cytogenetics | 2 H3 |
Refseq Size | 2752 |
Refseq ORF | 2121 |
Synonyms | 2310022K11Rik; C20orf67; F730014I05Rik |
Summary | Cap-specific adenosine methyltransferase that catalyzes formation of N(6),2'-O-dimethyladenosine cap (m6A(m)) by methylating the adenosine at the second transcribed position of capped mRNAs. Recruited to the early elongation complex of RNA polymerase II (RNAPII) via interaction with POLR2A and mediates formation of m6A(m) co-transcriptionally. N6-methylation of m6A(m) promotes the translation of capped mRNAs.[UniProtKB/Swiss-Prot Function] |
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