Misp (NM_030218) Mouse Recombinant Protein

CAT#: TP509752

Purified recombinant protein of Mouse mitotic spindle positioning (Misp), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR209752 representing NM_030218
  Red=Cloning site Green=Tags(s)
  
  MDRVTRYPIFSNPRSARVTSLVLDEDTSYTVELVGVGPEAGWSQGDLQAWSTEYQTRPDVKRTNVSSNRR
  VFLGQQSPRSLYLEDEDEEIKSYHLDDSSDTLCRQRRELEAERWAVIQSQAVRKGGTVATLQAASDHGDL
  RIARQPQSTVTEEILVDTEQIDFLAARQQFLSLEKANTNPVPCGLTARETFPRTPPGINQAPKASTGPHL
  ANGYTTAVTSPMKEVTLEKSFHVSPARSIHIVNDPGSQTQAESPETPKETPIEREIRLAQEREAELREQR
  GLGRAAGHQELVQIPSRPLLSKMSLTETPPRRDRGRPSLYVQRDMVQETQREEDHRREGLQVGRASTPDW
  PSQDPQPGLQRSLSSDCILSPDARATDPAPEARKVNRIPLDAYQPYLGPGTPKLEFSAFGVYSKPSGVST
  EDTKATVFQKATESPRHVSESSGRSLSSKQEWSKPPGKATNVGVVRLGNFHLRPLRFKVPDVPQGTETPH
  TWGWEVAGGPILRLQKSQSSDLLEREMESVLRREREVAEERRNAFFPEVFSPVPAEDESHEQDSRSSSRA
  SGITGSYSVSESPLFTPVHLNSGLVWKVEAPEDSAPPGQKTRKEMWYAGINPSDSVNSEVLGATRVKRHK
  NLLAERWEAHIYASEDEN
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 72.3 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_084494
• Locus ID: 78906
• UniProt ID: Q9D279
• Cytogenetics: 10 C1
• Refseq Size: 2427
• Refseq ORF: 1944
• Synonyms: 9130017N09Rik
• Summary: Plays a role in mitotic spindle orientation and mitotic progression. Regulates the distribution of dynactin at the cell cortex in a PLK1-dependent manner, thus stabilizing cortical and astral microtubule attachments required for proper mitotic spindle positioning. May link microtubules to the actin cytoskeleton and focal adhesions. May be required for directed cell migration and centrosome orientation. May also be necessary for proper stacking of the Golgi apparatus (By similarity).[UniProtKB/Swiss-Prot Function]