Inpp5e (NM_033134) Mouse Recombinant Protein

CAT#: TP509731

Purified recombinant protein of Mouse inositol polyphosphate-5-phosphatase E (Inpp5e), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR209731 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPSKSACLRHTEAPGQLEGRMLQGQPPNTEKKLIPTPGFLPASDSQGSETNPMPPFSIPAKTSNQNPQTK
  ANLITPQPPIRPKLERTLSLDDKGWRRRRFRGSQEDLTVQNGASPCRGSLQDSVAQSPAYSRPLPCLSTS
  LQEIPKSRRATGSEGGSPSLWSDCLSGMISTSLDLLHRDAASGGPPSRLASLHASHTPPAMDLSIASSSL
  RTANKVDPEHTDYKLRMQTRLVRAHSNLGPSRPRSPLAGDDHSIHSARSFSLLAPIRTKDIRSRSYLEGS
  LLASGALLGAEELARYFPDRNMALFVATWNMQGQKELPASLDEFLLPTEADYTQDLYVIGIQEGCSDRRE
  WETRLQETLGPQYVLLSSAAHGVLYMSLFIRRDLIWFCSEVEYSTVTTRIVSQIKTKGALGVSFTFFGTS
  FLFITSHFTSGDGKVAERLLDYSRTIQALALPRNVPDTNPYRSSAGDVTTRFDEVFWFGDFNFRLSGGRV
  AVEAFLKQKPEVDVLALLQHDQLTREMKKGSIFRGFEEAEIHFLPSYKFDIGKDTYDSTSKQRTPSYTDR
  VLYKSRHKGDICPMKYSSCPGIKTSDHRPVYGLFQVKVRPGRDNIPLAAGKFDRELYLIGIKRRISKEIQ
  RQEALKSQSSSAVCTVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 71.9 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_149125
• Locus ID: 64436
• UniProt ID: Q9JII1
• Cytogenetics: 2 A3
• Refseq Size: 4186
• Refseq ORF: 1944
• Synonyms: 72kDa; 1200002L24Rik; mKIAA0123
• Summary: Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3), phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Specific for lipid substrates, inactive towards water soluble inositol phosphates. Specific for lipid substrates, inactive towards water soluble inositol phosphates (By similarity) (PubMed:10806194). Plays an essential role in the primary cilium by controlling ciliary growth and phosphoinositide 3-kinase (PI3K) signaling and stability (PubMed:19668215).[UniProtKB/Swiss-Prot Function]