Ndst3 (BC079622) Mouse Recombinant Protein

CAT#: TP509708

Purified recombinant protein of Mouse N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (cDNA clone MGC:90637 IMAGE:30535990),, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR209708 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSFIMKPHRHFQRTLILLATFCMVSIIISAYYLYSGYKQESEVSGRASEVDCGDLQHIPSRLMEVRRTMI
  SDASRTDPTVLVFVESQYSSLGQDIIMMLESIRFHYHTEIAPGKGDLPALTDNVKGKYVLIIYENILKYI
  NMDSWNRSLLDKYCIEYGVGIIGFHKTSEKNLQSFQFRGFPFSISGNLAVKDCCINPHSPLLRVTKSSKL
  DRGSLPGTDWTVFQINHSTYQPVIFAKVKTPENLSPPISKHAFYATIIHDLGLHDGIQRVLFGNNLNFWL
  HKLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEGTRMNTNDVKALLDTQNLLRTQITNFTFNLGFS
  GKFYHTGTEEEDEGDDCLLGSVDEFWWFPHMWSHMQPHLFHNESSLIEQMILNKKFALEHGIPTDMGYAV
  SPHHSGVYPVHVQLYEAWKKVWNIKITSTEEYPHLKPARYRRGFIHKNIMVLPRQTCGLFTHTIFYKEYP
  GGPRELDKSIHGGELFFTVVLNPISIFMTHLSNYGNDRLGLYTFVNLANFVQTWTNLRLQTLPPAQLAHK
  YFELFPDQKDPLWQNPCDDKRHRDIWSKEKTCDRLPKFLVIGPQKTGTTALCLFLIMHPSILSNSPSPKS
  FEEVHGFLPSPI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 73.8 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• Locus ID: 83398
• UniProt ID: Q9EQH7
• Cytogenetics: 3 G1
• Refseq Size: 5317
• Refseq ORF: 1926
• Synonyms: 4921531K01Rik; 4930511P15Rik; N-HSST 3; NDST-3
• Summary: Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has high deacetylase activity but low sulfotransferase activity.[UniProtKB/Swiss-Prot Function]