Spast (NM_016962) Mouse Recombinant Protein

CAT#: TP509420

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Purified recombinant protein of Mouse spastin (Spast), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

USD 823.00

4 Weeks

    • 20 ug

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Product Data
Description Purified recombinant protein of Mouse spastin (Spast), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Species Mouse
Expression Host HEK293T
Expression cDNA Clone or AA Sequence A DNA sequence from Mouse cDNA ORF Clone, MR209420, encoding Mouse full-length Spast.
Predicted MW 66.8 kDa
Concentration >50 ug/mL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Storage Store at -80°C after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq NP_058658
Locus ID 50850
UniProt ID Q9QYY8, A0A286YE25
Cytogenetics 17 E2
Refseq Size 4693
Refseq ORF 1839
Synonyms mKIAA1083; Spg4
Summary ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated (PubMed:19141076 PubMed:20530212). Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (By similarity). Severing activity is not dependent on tubulin acetylation or detyrosination (By similarity). Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation (By similarity). It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia (By similarity). SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex (By similarity). Recruited at the midbody, probably by IST1, and participates in membrane fission during abscission together with the ESCRT-III complex (By similarity). Recruited to the nuclear membrane by IST1 and mediates microtubule severing, promoting nuclear envelope sealing and mitotic spindle disassembly during late anaphase (By similarity). Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and endosome recycling (By similarity). Recruited by IST1 to endosomes and regulates early endosomal tubulation and recycling by mediating microtubule severing (By similarity). Probably plays a role in axon growth and the formation of axonal branches (PubMed:18234839).[UniProtKB/Swiss-Prot Function]


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