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Scg2 (BC048249) Mouse Recombinant Protein

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SKU
TP509051
Purified recombinant protein of Mouse secretogranin II (cDNA clone MGC:54765 IMAGE:6493827), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Size:
20 ug
  $1,020.00
4 Weeks*
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Specifications
Specifications
Product Data
Species Mouse
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>MR209051 protein sequence
Red=Cloning site Green=Tags(s)

MAGAKAYRLGAVLLLIHLIFLISGAEAASFQRNQLLQKEPDLRLENVQKFPSPEMIRALEYIEKLRQQAH
REESSPDYNPYQGVSVPLQLKENGEESHLAESSRDALSEDEWMRIILEALRQAENEPPSAPKENKPYALN
LEKNFPVDTPDDYETQQWPERKLKHMRFPLMYEENSRENPFKRTNEIVEEQYTPQSLATLESVFQELGKL
TGPSNQKRERVDEEQKLYTDDEDDVYKTNNIAYEDVVGGEDWSPIEEKIETQTQEEVRDSKENTEKNEQI
NEEMKRSGQLGLPDEENRRESKDQLSEDASKVITYLRRNLQIPPEDLIEMLKAGEKPNGLVEPEQDLELA
VDLDDIPEADLDRPDMFQSKMLSKGGYPKAPGRGMVEALPDGLSVEDILNVLGMENVVNQKSPYFPNQYS
QDKALMRLPYGPGKSRANQIPKVAWIPDVESRQAPYENLNDQELGEYLARMLVKYPELLNTNQLKRVPSP
VSSEDDLQEEEQLEQAIKEHLGPGSSQEMERLAKVSKRIPVGSLKNEDTPNRQYLDEDMLLKVLEYLNQE
QAEQGREHLAKRAMENM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-MYC/DDK
Predicted MW 66.4 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
Locus ID 20254
UniProt ID Q03517
Cytogenetics 1 40.89 cM
RefSeq Size 2211
RefSeq ORF 1731
Synonyms Chgc; SgII
Summary Neuroendocrine protein of the granin family that regulates the biogenesis of secretory granules.UniProtKB/Swiss-Prot Function
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Documents
Resources

file-alt Citations

REST Regulates Non-Cell-Autonomous Neuronal Differentiation and Maturation of Neural Progenitor Cells via Secretogranin II
Kim, Denli, Wright et al
J. Neurosci. (2015) 35 (44), 14872-84 DOI: 10.1523/JNEUROSCI.4286-14.2015
AI Summary: OriGene contributed to this study by providing recombinant SCG2 protein, which was used to assess its role in rescuing neuronal differentiation phenotypes following REST knockdown.
Snippet: Cells under differentiation conditions were cultured for 3–6 d after addition of RA. Cells were transfected using Nucleofector (Amaxa). Recombinant SCG2 (MW 70.8 kDa, NP_003460) was purchased from Origene and used at 20 ng/ml, resuspended in 0.1% BSA in ... Cite SaveSaving
Showing 1-1 of 1 papers.
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