Sgpl1 (NM_009163) Mouse Recombinant Protein
SKU
TP508957
Purified recombinant protein of Mouse sphingosine phosphate lyase 1 (Sgpl1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
$1,020.00
4 Weeks*
Specifications
| Product Data | |
| Species | Mouse |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-MYC/DDK |
| Predicted MW | 63.7 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C after receiving vials. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_033189 |
| Locus ID | 20397 |
| UniProt ID | Q8R0X7 |
| Cytogenetics | 10 32.14 cM |
| RefSeq Size | 4133 |
| RefSeq ORF | 1704 |
| Synonyms | AI428538; D10Xrf456; S1PL; Spl |
| Summary | Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine (PubMed:9464245, PubMed:20097939). Elevates stress-induced ceramide production and apoptosis (PubMed:9464245). Required for global lipid homeostasis in liver and cholesterol homeostasis in fibroblasts (PubMed:20097939, PubMed:28262793). Involved in the regulation of pro-inflammatory response and neutrophil trafficking (PubMed:21173151). Modulates neuronal autophagy via phosphoethanolamine production which regulates accumulation of aggregate-prone proteins such as APP (PubMed:28521611). Seems to play a role in establishing neuronal contact sites and axonal maintenance (By similarity).UniProtKB/Swiss-Prot Function |
| Protein Categories | Growth Factors, Intracellular Proteins, Membrane Proteins |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
Site-Dependent Cysteine Lipidation Potentiates the Activation of Proapoptotic BAX
Cohen, Wales, McHenry et al
Cell Rep (2020) 30 (10), 3229-3239.e6 DOI: 10.1016/j.celrep.2020.02.057
Cohen, Wales, McHenry et al
Cell Rep (2020) 30 (10), 3229-3239.e6 DOI: 10.1016/j.celrep.2020.02.057
Cited by 33
AI Summary: OriGene’s SGPL1 Human Recombinant Protein (Cat# TP308705) was used in the study to investigate the enzymatic regulation of BAX activation and lipidation during apoptosis.
Snippet: PES, 1 mM EDTA, pH 7.4, complete protease inhibitor cocktail [Roche]) with mitochondria (1 μg/mL), BIM SAHBA2 (500 nM) or vehicle (0.05% DMSO), in the presence or absence of recombinant SGPL1 (1 mg, Origene), for 2 h at 37°C. Reactions were diluted in an equal volume of BAX FPLC buffer containing 2% CHAPS, followed by addition of propargyl hydrazine (1.25 μL of 10 mM solution) and 30 min incuba;Recombinant BAX WT Walensky Lab N/A Recombinant BAX C62A Walensky Lab N/A Recombinant BAX C126A Walensky Lab N/A SGPL1 Human Recombinant Protein Origene Cat# TP308705
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
