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Wee2 (NM_201370) Mouse Recombinant Protein

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SKU
TP508795
Purified recombinant protein of Mouse WEE1 homolog 2 (S. pombe) (Wee2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Size:
20 ug
  $1,020.00
4 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Mouse
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>MR208795 protein sequence
Red=Cloning site Green=Tags(s)

MADTETDQGLNKKLSFSFCEEDTESEGQMTAQDIGGAQSQKPGREESEELEGPVPPTRDELHTSLSRDKG
SPGPDLWKSVSGPPKCPETPVLHSKSKPPVPTNFSTPKNSLGQPEISLLEKLSSRSSRHMRLTPASLVDE
KTSLSLVNINPFTPETYRKLLLQSKGKRKTRDYLEETGEEESKSVQWLPAKRSILQETNMASRYEKEFFE
IEKIGVGEFGTVYKCIKRLDGCIYAIKRSAKSFSGLSNELDLHEVYAHAVLGHHPHVVRYYSSWIEDDHV
VIQNEYCNGGSLQAAISENTASNNHFQEPKLKDILLQISLGLKYIHNSGMVHLDIKPSNIFICHKMQCDS
PVGPEEAESEADWFLNASVMYKIGDLGHATSISKPKVEEGDTRFLANEILQENYQHLPKADIFALGLTIA
VAAGAESLPINGDMWHHIRKGNFPEISQELSDDFYGLLKNMIHPAPKERPSAAALARSRILWPFLEKTDE
LQKQLNLEKSKTATLKRELKKARHIQTPQREVHHCYYQICKGSRHLLVGGRRKAPSSFTRGTSSV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-MYC/DDK
Predicted MW 62.2 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_958758
Locus ID 381759
UniProt ID Q66JT0
Cytogenetics 6 B1
RefSeq Size 2886
RefSeq ORF 1665
Synonyms Gm1065; Wee1B
Summary Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.UniProtKB/Swiss-Prot Function
Protein Categories Enzyme: Kinases, Intracellular Proteins
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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