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Lipc (NM_008280) Mouse Recombinant Protein

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SKU
TP508201
Purified recombinant protein of Mouse lipase, hepatic (Lipc), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Size:
20 ug
  $1,020.00
4 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Mouse
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>MR208201 protein sequence
Red=Cloning site Green=Tags(s)

MGNPLQISIFLVFCIFIQSSACGQGVGTEPFGRSLGATEASKPLKKPETRFLLFQDENDRLGCRLRPQHP
ETLQECGFNSSQPLIMIIHGWSVDGLLENWIWKIVSALKSRQSQPVNVGLVDWISLAYQHYTIAVQNTRI
VGQDVAALLLWLEESAKFSRSKVHLIGYSLGAHVSGFAGSSMDGKNKIGRITGLDPAGPMFEGTSPNERL
SPDDANFVDAIHTFTREHMGLSVGIKQPIAHYDFYPNGGSFQPGCHFLELYKHIAEHGLNAITQTIKCAH
ERSVHLFIDSLQHSDLQSIGFQCSDMGSFSQGLCLSCKKGRCNTLGYDIRKDRSGKSKRLFLITRAQSPF
KVYHYQFKIQFINQIEKPVEPTFTMSLLGTKEEIKRIPITLGEGITSNKTYSFLITLDKDIGELILLKFK
WENSAVWANVWNTVQTIMLWGIEPHHSGLILKTIWVKAGETQQRMTFCPENLDDLQLHPSQEKVFVNCEV
KSKRLTESKEQMSQETHAKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-MYC/DDK
Predicted MW 57.4 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_032306
Locus ID 15450
UniProt ID P27656
Cytogenetics 9 39.57 cM
RefSeq Size 1934
RefSeq ORF 1530
Synonyms AI256194; HL; Hpl
Summary Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin.UniProtKB/Swiss-Prot Function
Protein Categories Secreated Proteins
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Documents
Resources

file-alt Citations

Downregulation of hepatic lipase is associated with decreased CD133 expression and clone formation in HepG2 cells
Liu, Zuo, Fang et al
Int. J. Mol. Med. (2018)
AI Summary: OriGene's pCMV6-Kan/Neo plasmid was used to clone the mouse LIPC gene, enabling the researchers to study the effects of LIPC downregulation on CD133 expression and clone formation in HepG2 cells.
Snippet: The mouse LIPC gene sequence (NM-008280) was amplified by PCR and cloned into the pCMV6-Kan/Neo plasmid (OriGene, Rockvile, MD, USA). A specific LIPC siRNA sequence was designed as: 5'-GGA GAA ACC CAG CAA AGA AdTdT-3' (10)
Showing 1-1 of 1 papers.
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