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Tcf4 (BC014293) Mouse Recombinant Protein

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SKU
TP508151
Purified recombinant protein of Mouse transcription factor 4 (cDNA clone MGC:13998 IMAGE:4014231), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Size:
20 ug
  $1,020.00
4 Weeks*
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Specifications
Specifications
Product Data
Species Mouse
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>MR208151 protein sequence
Red=Cloning site Green=Tags(s)

MYCAYTIPGMGGNSLMYYYNGKAVYAPSASTADYNRDSPGYPSSKPAASTFPSSFFMQDGHHSSDPWSSS
SGMNQPGYGGMLGNSSHIPQSSSYCSLHPHERLSYPSHSSADINSSLPPMSTFHRSGTNHYSTSSCTPPA
NGTDSIMANRGTGAAGSSQTGDALGKALASIYSPDHTNNSFSSNPSTPVGSPPSLSAGTAVWSRNGGQAS
SSPNYEGPLHSLQSRIEDRLERLDDAIHVLRNHAVGPSTAVPGGHGDMHGIMGPSHNGAMGSLGSGYGTS
LLSANRHSLMVGAHREDGVALRGSHSLLPNQVPVPQLPVQSATSPDLNPPQDPYRGMPPGLQGQSVSSGS
SEIKSDDEGDENLQDTKSSEDKKLDDDKKDIKSITSNNDDEDLTPEQKAEREKERRMANNARERLRVRDI
NEAFKELGRMVQLHLKSDKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVSSEPPPLSL
AGPHPGMGDAANHMGQM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-MYC/DDK
Predicted MW 54.1 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
Locus ID 21413
UniProt ID Q60722
Cytogenetics 18 E2
RefSeq Size 1765
RefSeq ORF 1521
Synonyms ME2, ITF-2, SEF-2, MITF-2A, MITF-2B, TFE, E2-2, E2.2, ITF2, SEF2, ASP-I2, ITF-2b, SEF2-1
Summary Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Isoform 2 inhibits MYOD1 activation of the cardiac alpha-actin promoter. Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription. May have a regulatory function in developmental processes as well as during neuronal plasticity.UniProtKB/Swiss-Prot Function
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Documents
Resources

file-alt Citations

Pdcd4 knockdown up-regulates MAP4K1 expression and activation of AP-1 dependent transcription through c-Myc
Wang, Zhang, Yang
Biochim. Biophys. Acta (2012) 1823 (10), 1807-14 DOI: 10.1016/j.bbamcr.2012.07.004
Cited by 44
AI Summary: OriGene contributed to this study by providing the wild-type Tcf4 cDNA, which was used to investigate the role of Tcf4 in MAP4K1 expression and AP-1 dependent transcription.
Snippet: The first 92 bp was deleted using QuickChange II XL Site-Directed Mutagenesis kit (Agilent Technologies) and sub-cloned into pcDNA4/HisMax vector (Life Technologies). The wild-type Tcf4 cDNA was purchased from Origene (Rockville, MD).
Showing 1-1 of 1 papers.
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