Bcat1 (NM_007532) Mouse Recombinant Protein
SKU
TP506032
Purified recombinant protein of Mouse branched chain aminotransferase 1, cytosolic (Bcat1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
$1,020.00
4 Weeks*
Specifications
| Product Data | |
| Species | Mouse |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-MYC/DDK |
| Predicted MW | 42.7 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C after receiving vials. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_031558 |
| Locus ID | 12035 |
| UniProt ID | P24288 |
| Cytogenetics | 6 77.27 cM |
| RefSeq Size | 7868 |
| RefSeq ORF | 1158 |
| Synonyms | BCATc; Eca39 |
| Summary | Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.UniProtKB/Swiss-Prot Function |
| Protein Categories | Intracellular Proteins |
Reviews
Documents
| FAQs |
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Resources
| Recombinant Protein Resources |
Citations
BCAT1 promotes osteoclast maturation by regulating branched-chain amino acid metabolism
Go, Shin, Jang et al
Exp Mol Med (2022) 54 (6), 825-833 DOI: 10.1038/s12276-022-00775-3
Go, Shin, Jang et al
Exp Mol Med (2022) 54 (6), 825-833 DOI: 10.1038/s12276-022-00775-3
Cited by 52
AI Summary: OriGene contributed to this study by providing si-Bcat2 and si-Control oligonucleotides, which were used to investigate the role of BCAT2 in osteoclast maturation.
Snippet: (Invitrogen, Waltham, MA, United States) according to manufacturers instructions. Serum-free opti-MEM (Gibco) was used to dilute the siRNA reagents. Si-Bcat2 and si-Control oligo were purchased from OriGene (Rockville, MD, USA). The mouse Bcat2 siRNA sequence was 5ʹ-GGAGUAGUUCGACAAAGUCUGCUGG-3ʹ. After 24 h, the cells were cultured with M-CSF (30 ng/ml) and RANKL (100 ng/ml) to induce thei
The BCAT1 CXXC Motif Provides Protection against ROS in Acute Myeloid Leukaemia Cells
Hillier, Allcott, Guest et al
Antioxidants (Basel) (2022) 11 (4) DOI: 10.3390/antiox11040683
Hillier, Allcott, Guest et al
Antioxidants (Basel) (2022) 11 (4) DOI: 10.3390/antiox11040683
Cited by 19
AI Summary: OriGene contributed to this study by providing the BCAT1 cDNA transcript variant 5 in the PCMV6-Entry vector (Cat# PS100001), which was used for site-directed mutagenesis and overexpression experiments to investigate the antioxidant role of the BCAT1 CXXC motif in acute myeloid leukaemia cells.
Snippet: cubated at 37 °C and 5% CO2. 2.2. BCAT1 Site Directed Mutagenesis and Retroviral Transduction of U937 Cells BCAT1 cDNA transcript variant 5 (NM_001178094) contained in PCMV6-Entry vector (PS100001, Origene) was obtained from Origene (Rockville, MD, USA). Site-directed mutagenesis of the BCAT1-CXXC motif was performed using the Quikchange II Site-directed Mutagenesis Kit (Agilent, UK) and mutagen
Showing 1-2 of 2 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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