Nans (NM_053179) Mouse Recombinant Protein

CAT#: TP505501

Purified recombinant protein of Mouse N-acetylneuraminic acid synthase (sialic acid synthase) (Nans), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR205501 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPLELELCPGRWVGGKHPCFIIAEIGQNHQGDIDVAKRMIRTAKECGADCAKFQKSELEFKFNRKALERP
  YTSKHSWGKTYGEHKRHLEFSHDQYKELQSYAQEIGIFFTASGMDEMAVEFLHELNVPFFKVGSGDTNNF
  PYLEKTAKKGRPMVISSGMQSMDTMKQVYQIVKPLNPNFCFLQCTSAYPLQPEDANLRVISEYQKLFPDI
  PIGYSGHETGIAISVAAVALGAKVLERHITLDKTWKGSDHSASLEPGELAELVRSVRLVERALGSPTKQL
  LPCEMACNEKLGKSVVAKVKIPAGTTLTLDMLTVKVGEPKGYPPEDIFNLAGKKVLVTIEEDDTVMEESV
  ESHSKKIKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 40.5 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_444409
• Locus ID: 94181
• UniProt ID: Q99J77
• Cytogenetics: 4 B1
• Refseq Size: 1904
• Refseq ORF: 1080
• Synonyms: 4632418E04Rik; BB056474; Sas
• Summary: Produces N-acetylneuraminate-9-phosphate (Neu5Ac-9-P) from N-acetylmannosamine 6-phosphate. Has no detectable activity towards N-acetylmannosamine or mannose 6-phosphate.[UniProtKB/Swiss-Prot Function]