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Mettl1 (NM_010792) Mouse Recombinant Protein

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SKU
TP503548
Purified recombinant protein of Mouse methyltransferase like 1 (Mettl1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Size:
20 ug
  $1,020.00
4 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Mouse
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>MR203548 protein sequence
Red=Cloning site Green=Tags(s)

MMAGAEAPQPQKRYYRQRAHSNPMADHTLRYPVKPEEMDWSELYPEFFAPLNQNKNHDDPKDEKEKHSGA
QVEFADIGCGYGGLLVALSPLFPDTLILGLEIRVKVSDYVQDRIRALRAAPGGGFQNIACLRSNAMKHLP
NFFRKGQLAKMFFLFPDPHFKRTKHKWRIISPTLLAEYAYVLRVGGLVYTVTDVPELHEWMCTHFEEHPL
FECVPLEELSEDPIVEHLGSSTEEGKKVLRNGGKNFPAVFRRIQDPLLQAVTPNPTLP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-MYC/DDK
Predicted MW 30.6 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_034922
Locus ID 17299
UniProt ID Q9Z120
Cytogenetics 10 D3
RefSeq Size 887
RefSeq ORF 804
Synonyms 2810012D02Rik
Summary Methyltransferase that mediates the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed:29983320). Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. Also acts as a methyltransferase for a subset of internal N(7)-methylguanine in mRNAs (PubMed:29983320). Internal N(7)-methylguanine methylation of mRNAs regulates translation (PubMed:29983320). Also methylates a specific subset of miRNAs, such as let-7. N(7)-methylguanine methylation of let-7 miRNA promotes let-7 miRNA processing by disrupting an inhibitory secondary structure within the primary miRNA transcript (pri-miRNA) (By similarity). Acts as a regulator of embryonic stem cell self-renewal and differentiation (PubMed:29983320).UniProtKB/Swiss-Prot Function
Protein Categories Intracellular Proteins
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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