Rab8a (NM_023126) Mouse Recombinant Protein
SKU
TP502203
Purified recombinant protein of Mouse RAB8A, member RAS oncogene family (Rab8a), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
$1,020.00
4 Weeks*
Specifications
| Product Data | |
| Species | Mouse |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-MYC/DDK |
| Predicted MW | 24.1 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C after receiving vials. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_075615 |
| Locus ID | 17274 |
| UniProt ID | P55258 |
| Cytogenetics | 8 34.84 cM |
| RefSeq Size | 2012 |
| RefSeq ORF | 621 |
| Synonyms | AA409338; Mel |
| Summary | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Plays an important role in ciliogenesis (By similarity). Together with MICALL2, may also regulate adherens junction assembly (PubMed:18094055). May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis (By similarity). Involved in autophagy (By similarity).UniProtKB/Swiss-Prot Function |
| Protein Categories | Intracellular Proteins |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
A Phosphosite Mutant Approach on LRRK2 Links Phosphorylation and Dephosphorylation to Protective and Deleterious Markers, Respectively
Marchand, Sarchione, Athanasopoulos et al
Cells (2022) 11 (6) DOI: 10.3390/cells11061018
Marchand, Sarchione, Athanasopoulos et al
Cells (2022) 11 (6) DOI: 10.3390/cells11061018
Cited by 7
AI Summary: OriGene's recombinant RAB8a protein was used as a substrate in kinase assays to assess the effects of LRRK2 phosphorylation site mutants on substrate phosphorylation in this study.
Snippet: d with phosphoric acid to remove free ATP. The paper discs were then air dried before scintillation counting. A total of 40 ng of purified LRRK2 proteins were mixed with 200 ng of recombinant RAB8a (OriGene, Rockville, MD, USA) and 100 µM of ATP in kinase buffer (Tris pH 7.4 25 mM, MgCl2 10 mM, DTT 2 mM, Triton X-100 0.02%) for 1 H at 37 °C. Phosphorylation of RAB8a was assay by SDS-PAGE. 2.6
Showing 1-1 of 1 papers.
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