Hsd17b12 (BC037620) Mouse Recombinant Protein
SKU
TP501559
Purified recombinant protein of Mouse hydroxysteroid (17-beta) dehydrogenase 12 (cDNA clone MGC:46848 IMAGE:5375831), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
$1,020.00
4 Weeks*
Specifications
| Product Data | |
| Species | Mouse |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-MYC/DDK |
| Predicted MW | 20.1 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C after receiving vials. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| Locus ID | 56348 |
| UniProt ID | O70503 |
| Cytogenetics | 2 E1 |
| RefSeq Size | 1808 |
| RefSeq ORF | 531 |
| Synonyms | KIK-I, Kik1 |
| Summary | Catalyzes the second of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme has a 3-ketoacyl-CoA reductase activity, reducing 3-ketoacyl-CoA to 3-hydroxyacyl-CoA, within each cycle of fatty acid elongation. Thereby, it may participate in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May also catalyze the transformation of estrone (E1) into estradiol (E2) and play a role in estrogen formation.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
Development of potential preclinical candidates with promising in vitro ADME profile for the inhibition of type 1 and type 2 17β-Hydroxysteroid dehydrogenases: Design, synthesis, and biological evaluation
Abdelsamie, Salah, Siebenbürger et al
Eur J Med Chem (2019) 178, 93-107 DOI: 10.1016/j.ejmech.2019.05.084
Abdelsamie, Salah, Siebenbürger et al
Eur J Med Chem (2019) 178, 93-107 DOI: 10.1016/j.ejmech.2019.05.084
AI Summary: In this study, OriGene’s recombinant mouse 17β-HSD1 cDNA was used to transiently express the enzyme in HEK293 cells for evaluating the inhibitory effects of potential drug candidates.
Snippet: Human and mouse 17β-HSD2 preparations were obtained by isolating the microsomal fractions of human placenta and mouse liver homogenates, respectively, according to the described methods [44]. Incubations were run with [3H]-E2 (human: 500 nM;mouse: 10 nM), cofactor NAD+, and inhibitor. Human 17β-HSD1 was prepared from the cytosolic fractions of human placenta, whereas recombinant mouse 17β-HSD1 cDN...
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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